OBJECTIVE: Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase. STUDY DESIGN: One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test. RESULTS: Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase. CONCLUSION: Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.
OBJECTIVE: Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase. STUDY DESIGN: One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test. RESULTS: Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase. CONCLUSION: Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.
Authors: Audrie A Medina-Colorado; Kathleen L Vincent; Aaron L Miller; Carrie A Maxwell; Lauren N Dawson; Trevelyn Olive; Elena V Kozlova; Marc M Baum; Richard B Pyles Journal: Anaerobe Date: 2017-04-26 Impact factor: 3.331
Authors: Sujatha Srinivasan; Matthew M Munch; Maria V Sizova; Tina L Fiedler; Christina M Kohler; Noah G Hoffman; Congzhou Liu; Kathy J Agnew; Jeanne M Marrazzo; Slava S Epstein; David N Fredricks Journal: J Infect Dis Date: 2016-08-15 Impact factor: 5.226
Authors: Jennifer M Fettweis; Myrna G Serrano; Philippe H Girerd; Kimberly K Jefferson; Gregory A Buck Journal: Chem Biodivers Date: 2012-05 Impact factor: 2.408