Literature DB >> 214422

Cell fractions and enzymatic activities of Ureaplasma urealyticum.

N Romano, R La Licata.   

Abstract

The localization of some enzymic activities in cell fractions of Ureaplasma urealyticum was studied. A quantitative evaluation of the effectiveness of several cell lysis procedures was obtained by using labeled membranes and sucrose density gradient centrifugation. Ultrasonic treatment was found to be the most effective procedure for lysing the cells, whereas digitonin and osmotic shock caused the lysis of only 70 and 50% of the cells, respectively. The localization of selected enzymes in Ureaplasma cells resembled that found in other Mycoplasma species. Adenosine triphosphatase, ribonuclease, deoxyribonuclease, and p-nitrophenylphosphatase activities were located exclusively in the membrane fraction, whereas urease and L-histidine ammonia-lyase were located in the cytoplasm.

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Year:  1978        PMID： 214422      PMCID： PMC218515          DOI： 10.1128/jb.136.3.833-838.1978

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  THE MOLECULAR BASIS OF HISTIDASE INDUCTION IN BACILLUS SUBTILIS.

Authors:  L H HARTWELL; B MAGASANIK
Journal:  J Mol Biol       Date:  1963-10       Impact factor: 5.469

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  L-histidine ammonia-lyase from a T-strain Mycoplasma (Ureaplasma urealyticum).

Authors:  F Ajello; N Romano; M F Massenti
Journal:  Boll Ist Sieroter Milan       Date:  1977-09

4.  Characterization of the mycoplasma membrane proteins. II. Solubilization and enzymic activities of Acholeplasma laidlawii membrane proteins.

Authors:  Z Ne'eman; I Kahane; S Razin
Journal:  Biochim Biophys Acta       Date:  1971-10-12

5.  Reassembly of Mycoplasma membranes disaggregated by detergents.

Authors:  S Rottem; O Stein; S Razin
Journal:  Arch Biochem Biophys       Date:  1968-04       Impact factor: 4.013

6.  Induction and repression of the histidine-degrading enzymes in Aerobacter aerogenes.

Authors:  B Magasanik; P Lund; F C Neidhardt; D T Schwartz
Journal:  J Biol Chem       Date:  1965-11       Impact factor: 5.157

7.  Localization of Enzymes in Mycoplasma.

Authors:  J D Pollack; S Razin; R C Cleverdon
Journal:  J Bacteriol       Date:  1965-09       Impact factor: 3.490

8.  Immunological analysis of plasma membranes of a T-strain of mycoplasma (Ureaplasma urealyticum).

Authors:  N Romano; R La Licata; G Scarlata
Journal:  Infect Immun       Date:  1977-06       Impact factor: 3.441

9.  Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma).

Authors:  G K Masover; S Razin; L Hayflick
Journal:  J Bacteriol       Date:  1977-04       Impact factor: 3.490

10.  Biosynthesis of saturated and unsaturated fatty acids by a T-strain mycoplasma (Ureaplasma).

Authors:  N Romano; S Rottem; S Razin
Journal:  J Bacteriol       Date:  1976-10       Impact factor: 3.490
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  6 in total

Review 1.  Microbial ureases: significance, regulation, and molecular characterization.

Authors:  H L Mobley; R P Hausinger
Journal:  Microbiol Rev       Date:  1989-03

2.  Enzymatic activities in cell fractions of mycoplasmalike organisms purified from aster yellows-infected plants.

Authors:  Y K Arora; R C Sinha
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

3.  Characteristics of Ureaplasma urealyticum urease.

Authors:  A Blanchard; S Razin; G E Kenny; M F Barile
Journal:  J Bacteriol       Date:  1988-06       Impact factor: 3.490

4.  Adenosine 5'-triphosphate synthesis induced by urea hydrolysis in Ureaplasma urealyticum.

Authors:  N Romano; G Tolone; F Ajello; R La Licata
Journal:  J Bacteriol       Date:  1980-11       Impact factor: 3.490

5.  Cloning of urease gene sequences from Providencia stuartii.

Authors:  H L Mobley; B D Jones; A E Jerse
Journal:  Infect Immun       Date:  1986-10       Impact factor: 3.441

6.  Growth inhibition of Ureaplasma urealyticum by the proton pump inhibitor lansoprazole: direct attribution to inhibition by lansoprazole of urease activity and urea-induced ATP synthesis in U. urealyticum.

Authors:  K Nagata; E Takagi; H Satoh; H Okamura; T Tamura
Journal:  Antimicrob Agents Chemother       Date:  1995-10       Impact factor: 5.191
  6 in total

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