AIM: To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP). METHODS: Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath. RESULTS: The agonists of tachykinin NK1 receptor (SP and [Sar9] SP), NK2 receptor (NKA and (β-Ala8)-NKA), and NK3 receptor (NKB and Senktide) all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060, NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atropine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122. CONCLUSION: Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore, SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.
AIM: To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP). METHODS:Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath. RESULTS: The agonists of tachykinin NK1 receptor (SP and [Sar9] SP), NK2 receptor (NKA and (β-Ala8)-NKA), and NK3 receptor (NKB and Senktide) all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060, NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atropine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122. CONCLUSION: Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore, SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.
Authors: C A Maggi; R M Catalioto; M Criscuoli; P Cucchi; S Giuliani; A Lecci; A Lippi; S Meini; R Patacchini; A R Renzetti; P Santicioli; M Tramontana; V Zagorodnyuk; A Giachetti Journal: Can J Physiol Pharmacol Date: 1997-06 Impact factor: 2.273
Authors: Heiko U De Schepper; Benedicte Y De Winter; Tom C Seerden; Arnold G Herman; Paul A Pelckmans; Joris G De Man Journal: Regul Pept Date: 2005-09-15
Authors: Sergio Gonzalo; Marta Sofía Valero; Fernando Martínez de Salinas; Claudia Vergara; María Pilar Arruebo; Miguel Ángel Plaza; María Divina Murillo; Laura Grasa Journal: Dig Dis Sci Date: 2014-11-05 Impact factor: 3.199