Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite.

A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08(T), was isolated from alpine glacier cryoconite. Growth of strain Cr6-08(T) occurred at 1-25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08(T) is most closely related to members of the genus Arthrobacter. Strain Cr6-08(T) possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys-L-Ala(4)), MK-9(H(2)) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C(15 : 0) and iso-C(15 : 0)) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08(T) was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain Cr6-08(T) is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08(T) ( = DSM 23324(T)  = LMG 26052(T)  = CGMCC 1.10698(T)).