Literature DB >> 21439122

Development of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) measurement method combined with SPE.

Yohei Inaba1, Satoshi Koide, Kenji Yokoyama, Isao Karube.   

Abstract

A novel separation method of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was developed. The C(18) and the strong cation-exchange (SCX) columns were employed to separate urinary 8-OHdG. The major interfering substances were removed by the consecutive processes of the C(18) and the SCX columns. This newly developed procedure allows researchers to quantitatively measure urinary 8-OHdG by high performance liquid chromatography-electrochemical detector (HPLC-ECD) successfully. The newly developed separation method produces the optimized procedure of 8-OHdG measurement, and followed by the evaluation of its basic performance. The optimized measurement was highly reproducible (CV=2.0-2.9%, n=10). A correlation was observed between the proposed HPLC-ECD method and the column switching method (r=0.96).

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Year:  2011        PMID： 21439122     DOI： 10.1093/chrsci/49.4.303

Source DB:  PubMed          Journal:  J Chromatogr Sci        ISSN： 0021-9665            Impact factor:   1.618


  7 in total

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Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2014-06-24       Impact factor: 4.254

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4.  The Concentration of 8-Hydroxy-2'-Deoxyguanosine in Plasma During the Menstrual Cycle in Young Japanese Women.

Authors:  Kaori Yama; Honoka Shinbo; Yuka Fujikane; Chiaki Mikami; Maiko Machida; Jun Miura
Journal:  Womens Health Rep (New Rochelle)       Date:  2022-02-22

Review 5.  8-oxoguanine and 8-oxodeoxyguanosine Biomarkers of Oxidative DNA Damage: A Review on HPLC-ECD Determination.

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6.  A micro-extraction technique using a new digitally controlled syringe combined with UHPLC for assessment of urinary biomarkers of oxidatively damaged DNA.

Authors:  Berta Mendes; Pedro Silva; Fernando Aveiro; Jorge Pereira; José S Câmara
Journal:  PLoS One       Date:  2013-03-06       Impact factor: 3.240

7.  Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users.

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Journal:  Saudi J Biol Sci       Date:  2020-03-12       Impact factor: 4.219
  7 in total

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