Characterization of the CD3 gamma and delta invariant subunits of the sheep T cell antigen receptor.

The CD3 gamma and delta chains of the sheep T cell antigen receptor have been characterized by cDNA cloning. In comparison to human and mouse protein sequences, short oligopeptide stretches have been deleted from both the gamma and delta chains. However, apart from further variability in the number and position of potential N-linked glycosylation sites, the general structure of the CD3 gamma and delta proteins is markedly conserved. Particularly remarkable are two motifs consisting of 8 and 32 amino acids, respectively, which locate in the C-terminal half of each protein and which have been precisely conserved in both the CD3 gamma and delta chains of all three species. These motifs probably contribute structural properties essential for the function of the CD3/T cell receptor complex. mRNA of the sheep CD3 gamma (1.5 and 1.2 kb) and CD3 delta (1.5 kb) chains are transcribed from closely linked, single-copy genes, suggesting that common mechanisms for the regulation of CD3 genes are also preserved between species.