Yuichi Miyabara1, Noriko Nishimura, Chiharu Tohyama. 1. Environmental Health Sciences Division, National Institute for Environmental Studies (NIES), Tsukuba, Japan, miyabar@gipac.shinshu-u.ac.jp.
Abstract
OBJECTIVES: To clarify the origin of dioxin and related compounds (dioxins) in human hair, we determined the amounts of adsorbed dioxins in human hair, and the distribution of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rats. METHODS: Human hair specimens, packed in a glass column, were exposed to ambient air that was introduced into the column with an air pump for 24 h. Rats were administered TCDD by gavage at doses of 0.2, 0.8, and 1.6 μg/kg body weight. Four weeks after TCDD administration, hair from the back, serum, and adipose tissue were removed under diethyl ether anesthesia. The amounts of dioxins in these samples were analyzed by high resolution gas chromatography with mass spectroscopy. RESULTS: Exposure of the hair specimens to ambient air for one day increased the total toxic equivalent (TEQ) value by 51%. In TCDD-treated rats, the amount of TCDD in hair increased in a dose-dependent manner, and showed a significant positive correlation with that in adipose tissue. CONCLUSIONS: Human hair was found to retain dioxins by both internal and external exposure, and the contribution of external exposure was estimated to be about 40% of the TEQ.
OBJECTIVES: To clarify the origin of dioxin and related compounds (dioxins) in human hair, we determined the amounts of adsorbed dioxins in human hair, and the distribution of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rats. METHODS:Human hair specimens, packed in a glass column, were exposed to ambient air that was introduced into the column with an air pump for 24 h. Rats were administered TCDD by gavage at doses of 0.2, 0.8, and 1.6 μg/kg body weight. Four weeks after TCDD administration, hair from the back, serum, and adipose tissue were removed under diethyl ether anesthesia. The amounts of dioxins in these samples were analyzed by high resolution gas chromatography with mass spectroscopy. RESULTS: Exposure of the hair specimens to ambient air for one day increased the total toxic equivalent (TEQ) value by 51%. In TCDD-treated rats, the amount of TCDD in hair increased in a dose-dependent manner, and showed a significant positive correlation with that in adipose tissue. CONCLUSIONS:Human hair was found to retain dioxins by both internal and external exposure, and the contribution of external exposure was estimated to be about 40% of the TEQ.
Entities:
Keywords:
absorption; accelerated solvent extractor (ASE); air monitoring; dioxins; human hair
Authors: T Ohgami; S Nonaka; F Murayama; K Yamashita; H Irifune; M Watanabe; N Tsukazaki; K Tanaka; H Yoshida; Y Rikioka Journal: Fukuoka Igaku Zasshi Date: 1989-05
Authors: M Van den Berg; L Birnbaum; A T Bosveld; B Brunström; P Cook; M Feeley; J P Giesy; A Hanberg; R Hasegawa; S W Kennedy; T Kubiak; J C Larsen; F X van Leeuwen; A K Liem; C Nolt; R E Peterson; L Poellinger; S Safe; D Schrenk; D Tillitt; M Tysklind; M Younes; F Waern; T Zacharewski Journal: Environ Health Perspect Date: 1998-12 Impact factor: 9.031