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Literature DB >> 21431687

Identification of viral suppressors of RNAi by a reporter assay in Drosophila S2 cell culture.

Koen W R van Cleef¹, Joël T van Mierlo, Marius van den Beek, Ronald P van Rij.

Abstract

The RNA interference (RNAi) pathway plays an important role in antiviral immunity in insects. To -counteract the RNAi-mediated immune response of their hosts, several insect viruses, such as Flock house virus, Drosophila C virus, and Cricket paralysis virus, encode potent viral suppressors of RNAi (VSRs). Because of the importance of RNAi in antiviral defense in insects, other insect viruses are likely to encode VSRs as well. In this chapter, we describe a detailed protocol for an RNAi reporter assay in Drosophila S2 cells for the identification of VSR activity.

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Year: 2011 PMID： 21431687 DOI： 10.1007/978-1-61779-037-9_12

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

19 in total

1. Induction and Suppression of NF-κB Signalling by a DNA Virus of Drosophila.

Authors: William H Palmer; Joep Joosten; Gijs J Overheul; Pascal W Jansen; Michiel Vermeulen; Darren J Obbard; Ronald P Van Rij
Journal: J Virol Date: 2019-01-17 Impact factor: 5.103

2. Adventitious viruses persistently infect three commonly used mosquito cell lines.

Authors: James Weger-Lucarelli; Claudia Rückert; Nathan D Grubaugh; Michael J Misencik; Philip M Armstrong; Mark D Stenglein; Gregory D Ebel; Doug E Brackney
Journal: Virology Date: 2018-06-26 Impact factor: 3.616

3. Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor.

Authors: Pavan Kumar Kakumani; Sanket Singh Ponia; Rajgokul K S; Vikas Sood; Mahendran Chinnappan; Akhil C Banerjea; Guruprasad R Medigeshi; Pawan Malhotra; Sunil K Mukherjee; Raj K Bhatnagar
Journal: J Virol Date: 2013-06-05 Impact factor: 5.103

4. The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery.

Authors: Alfred W Bronkhorst; Koen W R van Cleef; Nicolas Vodovar; Ikbal Agah Ince; Hervé Blanc; Just M Vlak; Maria-Carla Saleh; Ronald P van Rij
Journal: Proc Natl Acad Sci U S A Date: 2012-11-14 Impact factor: 11.205

5. Investigating Virus-Host Interactions in Cultured Primary Honey Bee Cells.

Authors: Alexander J McMenamin; Fenali Parekh; Verena Lawrence; Michelle L Flenniken
Journal: Insects Date: 2021-07-17 Impact factor: 2.769

6. A unique nodavirus with novel features: mosinovirus expresses two subgenomic RNAs, a capsid gene of unknown origin, and a suppressor of the antiviral RNA interference pathway.

Authors: Susan Schuster; Florian Zirkel; Andreas Kurth; Koen W R van Cleef; Christian Drosten; Ronald P van Rij; Sandra Junglen
Journal: J Virol Date: 2014-09-10 Impact factor: 5.103

Identification of viral suppressors of RNAi by a reporter assay in Drosophila S2 cell culture.

1. Induction and Suppression of NF-κB Signalling by a DNA Virus of Drosophila.

2. Adventitious viruses persistently infect three commonly used mosquito cell lines.

3. Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor.

4. The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery.

5. Investigating Virus-Host Interactions in Cultured Primary Honey Bee Cells.

6. A unique nodavirus with novel features: mosinovirus expresses two subgenomic RNAs, a capsid gene of unknown origin, and a suppressor of the antiviral RNA interference pathway.

7. Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells.

8. RNA interference targets arbovirus replication in Culicoides cells.

9. Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses.

10. The heat shock response restricts virus infection in Drosophila.