| Literature DB >> 21431524 |
Abstract
Mesenchymal stem cells have the capability to differentiate into a number of cell types including adipocytes. The adipocytic phenotype is characterized by intracellular accumulation of lipid droplets as well as transcription of adipocyte-specific genes. This paper details a basic protocol for adipogenic induction of bone marrow and adipose tissue-derived stem cells, as well as protocols for staining lipid accumulation and the transcriptional analysis of PPAR-γ and aP2 by real-time RT-PCR.Entities:
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Year: 2011 PMID: 21431524 DOI: 10.1007/978-1-60761-999-4_19
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745