S Peterss1, N Khaladj, M Pichlmaier, K Hoeffler, R von Wasielewski, M L Shrestha, A Haverich, C Hagl.
Abstract
BACKGROUND: To avoid extended cardiopulmonary bypass (CPB), moderate temperatures are commonly accepted for hypothermic circulatory arrest (HCA), thereby jeopardizing organ protection. Distal aortic perfusion may be an option, but supportive experimental data is missing.
METHODS: Eight juvenile pigs (36 ± 2 kg) were cooled to 30 °C followed by 60 min of HCA with 50 min of low flow (LF) lower body perfusion. Multimodal monitoring was used to measure overall metabolism, hemodynamics and microcirculation of the terminal ileum. The animals were observed for four hours following reperfusion. Organs were harvested for histopathological evaluation.
RESULTS: During LF perfusion, initially elevated l-lactate levels decreased subsequently ( P < 0.05). Capillary blood flow decreased during cooling to 50 % baseline levels ( P = 0.03), but remained stable under LF conditions. Parameters indicative of reduced liver and kidney function were slightly elevated at the end of the experiment, but still within normal ranges.
CONCLUSION: Under moderate hypothermia, low flow perfusion seems to provide adequate protection for the lower body organs. Microcirculatory parameters during perfusion as well as lactate levels within normal ranges throughout the experiments further confirm the concept. © Georg Thieme Verlag KG Stuttgart · New York.
BACKGROUND: To avoid extended cardiopulmonary bypass (CPB), moderate temperatures are commonly accepted for hypothermic circulatory arrest (HCA), thereby jeopardizing organ protection. Distal aortic perfusion may be an option, but supportive experimental data is missing.
METHODS: Eight juvenile pigs (36 ± 2 kg) were cooled to 30 °C followed by 60 min of HCA with 50 min of low flow (LF) lower body perfusion. Multimodal monitoring was used to measure overall metabolism, hemodynamics and microcirculation of the terminal ileum. The animals were observed for four hours following reperfusion. Organs were harvested for histopathological evaluation.
RESULTS: During LF perfusion, initially elevated l-lactate levels decreased subsequently ( P < 0.05). Capillary blood flow decreased during cooling to 50 % baseline levels ( P = 0.03), but remained stable under LF conditions. Parameters indicative of reduced liver and kidney function were slightly elevated at the end of the experiment, but still within normal ranges.
CONCLUSION: Under moderate hypothermia, low flow perfusion seems to provide adequate protection for the lower body organs. Microcirculatory parameters during perfusion as well as lactate levels within normal ranges throughout the experiments further confirm the concept. © Georg Thieme Verlag KG Stuttgart · New York.
Entities:
Mesh：
Substances：
Year: 2011
PMID： 21425052 DOI： 10.1055/s-0030-1250727
Source DB: PubMed Journal: Thorac Cardiovasc Surg ISSN： 0171-6425 Impact factor: 1.827