Literature DB >> 2141979

Rat liver NAD(P)H:quinone oxidoreductase: cDNA expression and site-directed mutagenesis.

G L Forrest1, J Qian, J X Ma, W D Kaplan, S Akman, J Doroshow, S A Chen.   

Abstract

Rat liver NAD(P)H:quinone oxidoreductase cDNA was cloned and expressed in a eukaryotic cell expression plasmid containing a cytomegalovirus (CMV) promoter. Transient expression of enzyme activity and RNA transcription were measured in COS7 cells. The expressed quinone reductase has kinetic properties similar to the rat liver enzyme and is inhibited by dicourmarol, a known inhibitor of NAD(P)H:quinone oxidoreductase. Site-directed mutagenesis experiments carried out using this expression system revealed possible regions involved in NAD(P)H binding.

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Year:  1990        PMID： 2141979     DOI： 10.1016/0006-291x(90)92006-l

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  4 in total

1.  Expression of rat liver NAD(P)H:quinone-acceptor oxidoreductase in Escherichia coli and mutagenesis in vitro at Arg-177.

Authors:  H H Chen; J X Ma; G L Forrest; P S Deng; P A Martino; T D Lee; S Chen
Journal:  Biochem J       Date:  1992-06-15       Impact factor: 3.857

2.  The role of NAD(P)H:quinone oxidoreductase in mitomycin C- and porfiromycin-resistant HCT 116 human colon-cancer cells.

Authors:  S S Pan; S A Akman; G L Forrest; C Hipsher; R Johnson
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

3.  A two-domain structure for the two subunits of NAD(P)H:quinone acceptor oxidoreductase.

Authors:  S Chen; P S Deng; J M Bailey; K M Swiderek
Journal:  Protein Sci       Date:  1994-01       Impact factor: 6.725

4.  Mouse liver NAD(P)H:quinone acceptor oxidoreductase: protein sequence analysis by tandem mass spectrometry, cDNA cloning, expression in Escherichia coli, and enzyme activity analysis.

Authors:  S Chen; P E Clarke; P A Martino; P S Deng; C H Yeh; T D Lee; H J Prochaska; P Talalay
Journal:  Protein Sci       Date:  1994-08       Impact factor: 6.725
  4 in total

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