BACKGROUND: Many experimental models of acute pancreatitis suffer from lack of clinical relevance. We sought to validate a recently reported murine model of acute pancreatitis that more closely represents the physiology of human biliary pancreatitis. METHODS: Mice (C57BL/6J n=6 and CF-1 n=8) underwent infusion of 50μl of 5% sodium taurocholate (NaT) or 50μl of normal saline (NaCl) directly into the pancreatic duct. Twenty-four hours later, pancreatitis severity was graded histologically by three independent observers, and pancreatic tissue concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined by ELISA. RESULTS: Twenty four hours after retrograde injection, the total pancreatitis score was significantly greater in mice infused with NaT than in those infused with NaCl (6.3 ± 1.2 vs. 1.2 ± 0.4, p<0.05). In addition, the inflammatory mediators IL-6 and MCP-1 were increased in the NaT group relative to the NaCl group. DISCUSSION: Retrograde pancreatic duct infusion of sodium taurocholate induces acute pancreatitis in the mouse. This model is likely representative of human biliary pancreatitis pathophysiology, and therefore provides a powerful tool with which to elucidate basic mechanisms underlying the pathogenesis of acute pancreatitis.
BACKGROUND: Many experimental models of acute pancreatitis suffer from lack of clinical relevance. We sought to validate a recently reported murine model of acute pancreatitis that more closely represents the physiology of human biliary pancreatitis. METHODS:Mice (C57BL/6J n=6 and CF-1 n=8) underwent infusion of 50μl of 5% sodium taurocholate (NaT) or 50μl of normal saline (NaCl) directly into the pancreatic duct. Twenty-four hours later, pancreatitis severity was graded histologically by three independent observers, and pancreatic tissue concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined by ELISA. RESULTS: Twenty four hours after retrograde injection, the total pancreatitis score was significantly greater in mice infused with NaT than in those infused with NaCl (6.3 ± 1.2 vs. 1.2 ± 0.4, p<0.05). In addition, the inflammatory mediators IL-6 and MCP-1 were increased in the NaT group relative to the NaCl group. DISCUSSION: Retrograde pancreatic duct infusion of sodium taurocholate induces acute pancreatitis in the mouse. This model is likely representative of human biliary pancreatitis pathophysiology, and therefore provides a powerful tool with which to elucidate basic mechanisms underlying the pathogenesis of acute pancreatitis.
Authors: Bettina Rau; Katja Baumgart; Colin M Krüger; Martin Schilling; Hans G Beger Journal: Intensive Care Med Date: 2003-02-15 Impact factor: 17.440
Authors: Nicholas J Zyromski; Abhishek Mathur; Henry A Pitt; Debao Lu; John T Gripe; Julia J Walker; Kyle Yancey; Terence E Wade; Deborah A Swartz-Basile Journal: Am J Physiol Gastrointest Liver Physiol Date: 2008-06-26 Impact factor: 4.052
Authors: Nils Habbe; Guanglu Shi; Robert A Meguid; Volker Fendrich; Farzad Esni; Huiping Chen; Georg Feldmann; Doris A Stoffers; Stephen F Konieczny; Steven D Leach; Anirban Maitra Journal: Proc Natl Acad Sci U S A Date: 2008-11-21 Impact factor: 11.205
Authors: Alexandra M Roch; Thomas K Maatman; Todd G Cook; Howard H Wu; Stephanie Merfeld-Clauss; Dmitry O Traktuev; Keith L March; Nicholas J Zyromski Journal: J Gastrointest Surg Date: 2019-11-19 Impact factor: 3.452
Authors: Hayder H Al-Azzawi; Kathryn M Ziegler; Deborah A Swartz-Basile; Sue Wang; Henry A Pitt; Nicholas J Zyromski Journal: J Gastrointest Surg Date: 2011-06-10 Impact factor: 3.452
Authors: Mei H Wan; Wei Huang; Diane Latawiec; Kun Jiang; David M Booth; Victoria Elliott; Rajarshi Mukherjee; Qing Xia Journal: HPB (Oxford) Date: 2012-02 Impact factor: 3.647