SUMMARY: BACKGROUND: In a previous publication we described a method for Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, K/k, Kp(a)/Kp(b), Js(a)/Js(b), Co(a)/Co(b), and Lu(a)/Lu(b) genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. METHODS: TWO MULTIPLEX POLYMERASE CHAIN REACTIONS (PCR) WERE DEVELOPED: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. RESULTS: There have been no discrepancies with the serology results other than null and/or weak phenotypes. CONCLUSION: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
SUMMARY: BACKGROUND: In a previous publication we described a method for Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, K/k, Kp(a)/Kp(b), Js(a)/Js(b), Co(a)/Co(b), and Lu(a)/Lu(b) genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. METHODS: TWO MULTIPLEX POLYMERASE CHAIN REACTIONS (PCR) WERE DEVELOPED: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. RESULTS: There have been no discrepancies with the serology results other than null and/or weak phenotypes. CONCLUSION: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
Authors: Ghazala Hashmi; Tasmia Shariff; Yi Zhang; Joan Cristobal; Chiu Chau; Michael Seul; Prabhakar Vissavajjhala; Christopher Baldwin; Kim Hue-Roye; Dalisay Charles-Pierre; Christine Lomas-Francis; Marion E Reid Journal: Transfusion Date: 2007-04 Impact factor: 3.157
Authors: Neil D Avent; Antonio Martinez; Willy A Flegel; Martin L Olsson; Marion L Scott; Núria Nogués; Martin Písăcka; Geoff Daniels; Ellen van der Schoot; Eduardo Muñiz-Diaz; Tracey E Madgett; Jill R Storry; Sigrid H Beiboer; Petra A Maaskant-van Wijk; Inge von Zabern; Elisa Jiménez; Diego Tejedor; Mónica López; Emma Camacho; Goedele Cheroutre; Anita Hacker; Pavel Jinoch; Irena Svobodova; Masja de Haas Journal: Transfusion Date: 2007-07 Impact factor: 3.157
Authors: K Karpasitou; S Frison; E Longhi; F Drago; R Lopa; F Truglio; M Marini; S Bresciani; M Scalamogna; F Poli Journal: Vox Sang Date: 2010-03-21 Impact factor: 2.144