| Literature DB >> 21413172 |
Omar Qazi1, Mridula Rani, Annie J Gnanam, Thomas W Cullen, Christopher M Stead, Haley Kensing, Kate McCaul, Sarah Ngugi, Joann L Prior, Alexandria Lipka, Judit M Nagy, C Whitlock Gregory, Barbara M Judy, Sarah V Harding, Richard W Titball, Sachdev S Sidhu, M Stephen Trent, G Barrie Kitto, Alfredo Torres, D Mark Estes, Brent Iverson, George Georgiou, Katherine A Brown.
Abstract
Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.Entities:
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Year: 2011 PMID： 21413172 PMCID： PMC3593192 DOI： 10.1039/c005422b
Source DB: PubMed Journal: Faraday Discuss ISSN： 1359-6640 Impact factor: 4.008