Literature DB >> 214125

A rapid purification of T4 polynucleotide kinase using Blue Dextran-Sepharose chromatography.

B P Nichols, T D Lindell, E Stellwagen, J E Donelson.   

Abstract

A rapid batch procedure is described for purification of T4 polynucleotide kinase (ATP:5'-dephosphopolynucleotide 5'-phosphotransferase, EC 2.7.1.78) to near homogeneity using Blue Dextran-Sepharose chromatography. The enzyme preparation is sufficiently free of contaminating endonuclease and alkaline phosphatase activities to be suitable for radioactively labeling nucleic acids in vitro. Kinetic measurements indicate that the chromophore of Blue Dextran, Cibacron Blue F3GA, inhibits the activity of T4 polynucleotide kinase competitively with respect to single stranded DNA substrate and non-competitively with respect to the rATP substrate.

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Year:  1978        PMID： 214125     DOI： 10.1016/0005-2744(78)90132-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  2 in total

1.  Effect of Cibacron blue F3GA on oligonucleotide binding site of estradiol--receptor complexes of mouse uterine cytosol.

Authors:  S A Kumar; T A Beach; H W Dickerman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

2.  A new approach to the synthesis of the 5'-end substituted oligonucleotides using T4 polynucleotide kinase and gamma-amides of ATP bearing photoreactive groups.

Authors:  I O Petrousseva; I V Safronov; N I Komarova; T P Kamynina; O I Lavrik; S N Khodyreva
Journal:  Dokl Biochem Biophys       Date:  2003 Mar-Apr       Impact factor: 0.788
  2 in total

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