Literature DB >> 21412270

Transient expression and analysis of fluorescent reporter proteins in plant pollen tubes.

Hao Wang1, Liwen Jiang.   

Abstract

The pollen tube is an excellent single-cell model system for studying cellular processes in plant cell biology. This protocol describes a detailed step-by-step procedure with optimized conditions for introducing various fluorescent reporter proteins into lily, tobacco and Arabidopsis pollen grains by means of biolistics for their transient expression and subsequent analysis in germinating pollen tubes. The whole experiment consists of four major stages: coating gold microcarriers with DNA constructs, preparation of pollen grains, transformation of plasmid DNA into pollen grains by particle delivery system and germination of bombarded pollen grains in optimized germination media to obtain pollen tubes for protein trafficking, protein localization, drug treatment and organelle dynamics analysis. This protocol takes about 4-12 h from pollen preparation to protein detection.

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Year:  2011        PMID： 21412270     DOI： 10.1038/nprot.2011.309

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  48 in total

Review 1.  How pollen tubes grow.

Authors:  Alexander Krichevsky; Stanislav V Kozlovsky; Guo-Wei Tian; Min-Huei Chen; Adi Zaltsman; Vitaly Citovsky
Journal:  Dev Biol       Date:  2006-12-08       Impact factor: 3.582

2.  Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.

Authors:  Sang-Dong Yoo; Young-Hee Cho; Jen Sheen
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

3.  The vacuolar transport of aleurain-GFP and 2S albumin-GFP fusions is mediated by the same pre-vacuolar compartments in tobacco BY-2 and Arabidopsis suspension cultured cells.

Authors:  Yansong Miao; Kwun Yee Li; Hong-Ye Li; Xiaoqiang Yao; Liwen Jiang
Journal:  Plant J       Date:  2008-09-04       Impact factor: 6.417

4.  Interdependence of endomembrane trafficking and actin dynamics during polarized growth of Arabidopsis pollen tubes.

Authors:  Yan Zhang; Junmin He; David Lee; Sheila McCormick
Journal:  Plant Physiol       Date:  2010-02-24       Impact factor: 8.340

5.  A protocol for Agrobacterium-mediated transformation in rice.

Authors:  Asuka Nishimura; Ikuko Aichi; Makoto Matsuoka
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Localization of green fluorescent protein fusions with the seven Arabidopsis vacuolar sorting receptors to prevacuolar compartments in tobacco BY-2 cells.

Authors:  Yansong Miao; Pak Kan Yan; Hyeran Kim; Inhwan Hwang; Liwen Jiang
Journal:  Plant Physiol       Date:  2006-09-15       Impact factor: 8.340

7.  Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells.

Authors:  Yu Chung Tse; Beixin Mo; Stefan Hillmer; Min Zhao; Sze Wan Lo; David G Robinson; Liwen Jiang
Journal:  Plant Cell       Date:  2004-02-18       Impact factor: 11.277

8.  An exocyst complex functions in plant cell growth in Arabidopsis and tobacco.

Authors:  Michal Hála; Rex Cole; Lukás Synek; Edita Drdová; Tamara Pecenková; Alfred Nordheim; Tobias Lamkemeyer; Johannes Madlung; Frank Hochholdinger; John E Fowler; Viktor Zárský
Journal:  Plant Cell       Date:  2008-05-20       Impact factor: 11.277

9.  Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.

Authors:  Luis Vidali; Caleb M Rounds; Peter K Hepler; Magdalena Bezanilla
Journal:  PLoS One       Date:  2009-05-29       Impact factor: 3.240

10.  Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth.

Authors:  Yong Jik Lee; Amy Szumlanski; Erik Nielsen; Zhenbiao Yang
Journal:  J Cell Biol       Date:  2008-06-30       Impact factor: 10.539
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  22 in total

Review 1.  Secretory carrier membrane proteins.

Authors:  Angus Ho Yin Law; Cheung-Ming Chow; Liwen Jiang
Journal:  Protoplasma       Date:  2011-06-02       Impact factor: 3.356

2.  De novo sequencing and analysis of the lily pollen transcriptome: an open access data source for an orphan plant species.

Authors:  Veronika Lang; Björn Usadel; Gerhard Obermeyer
Journal:  Plant Mol Biol       Date:  2014-10-24       Impact factor: 4.076

3.  Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants.

Authors:  Xiaomin Peng; Guitao Zhong; Hao Wang
Journal:  J Vis Exp       Date:  2018-07-01       Impact factor: 1.355

4.  A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation.

Authors:  Hao Wang; Xiaohong Zhuang; Xiangfeng Wang; Angus Ho Yin Law; Teng Zhao; Shengwang Du; Michael M T Loy; Liwen Jiang
Journal:  Plant Physiol       Date:  2016-08-16       Impact factor: 8.340

5.  A Glycosyltransferase from Nicotiana alata Pollen Mediates Synthesis of a Linear (1,5)-α-L-Arabinan When Expressed in Arabidopsis.

Authors:  Edwin R Lampugnani; Yin Ying Ho; Isabel E Moller; Poh-Ling Koh; John F Golz; Antony Bacic; Ed Newbigin
Journal:  Plant Physiol       Date:  2016-02-05       Impact factor: 8.340

6.  Trans-Golgi network-located AP1 gamma adaptins mediate dileucine motif-directed vacuolar targeting in Arabidopsis.

Authors:  Xiangfeng Wang; Yi Cai; Hao Wang; Yonglun Zeng; Xiaohong Zhuang; Baiying Li; Liwen Jiang
Journal:  Plant Cell       Date:  2014-10-28       Impact factor: 11.277

7.  Tomato Pistil Factor STIG1 Promotes in Vivo Pollen Tube Growth by Binding to Phosphatidylinositol 3-Phosphate and the Extracellular Domain of the Pollen Receptor Kinase LePRK2.

Authors:  Wei-Jie Huang; Hai-Kuan Liu; Sheila McCormick; Wei-Hua Tang
Journal:  Plant Cell       Date:  2014-06-17       Impact factor: 11.277

8.  Cis-Regulatory Elements Determine Germline Specificity and Expression Level of an Isopentenyltransferase Gene in Sperm Cells of Arabidopsis.

Authors:  Jinghua Zhang; Tong Yuan; Xiaomeng Duan; Xiaoping Wei; Tao Shi; Jia Li; Scott D Russell; Xiaoping Gou
Journal:  Plant Physiol       Date:  2016-01-06       Impact factor: 8.340

9.  Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth.

Authors:  Gang Li; Xiujuan Yang; Xiaoqing Zhang; Yu Song; Wanqi Liang; Dabing Zhang
Journal:  Plant Physiol       Date:  2018-03-26       Impact factor: 8.340

Review 10.  Male functions and malfunctions: the impact of phosphoinositides on pollen development and pollen tube growth.

Authors:  Ingo Heilmann; Till Ischebeck
Journal:  Plant Reprod       Date:  2015-12-16       Impact factor: 3.767
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