Literature DB >> 21409630

Encapsulation of cells in agarose beads.

J Overhauser1.   

Abstract

For the analysis of large DNA fragments using pulsed-field gel electrophoresis (PFGE), it is necessary to first embed the cells in agarose to prevent shearing of the DNA during protein extraction and restriction enzyme digestion. The speed and efficiency with which these steps are performed is based on the diffusion rate of the buffers and proteins in each step.

Entities:  

Year:  1992        PMID： 21409630     DOI： 10.1385/0-89603-229-9:129

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  One-step microfluidic generation of pre-hatching embryo-like core-shell microcapsules for miniaturized 3D culture of pluripotent stem cells.

Authors:  Pranay Agarwal; Shuting Zhao; Peter Bielecki; Wei Rao; Jung Kyu Choi; Yi Zhao; Jianhua Yu; Wujie Zhang; Xiaoming He
Journal:  Lab Chip       Date:  2013-12-07       Impact factor: 6.799

2.  The effect of RGD peptide on 2D and miniaturized 3D culture of HEPM cells, MSCs, and ADSCs with alginate hydrogel.

Authors:  Jenna Dumbleton; Pranay Agarwal; Haishui Huang; Nathaniel Hogrebe; Renzhi Han; Keith J Gooch; Xiaoming He
Journal:  Cell Mol Bioeng       Date:  2016-01-26       Impact factor: 2.321

3.  ecDNA hubs drive cooperative intermolecular oncogene expression.

Authors:  King L Hung; Kathryn E Yost; Liangqi Xie; Quanming Shi; Konstantin Helmsauer; Jens Luebeck; Robert Schöpflin; Joshua T Lange; Rocío Chamorro González; Natasha E Weiser; Celine Chen; Maria E Valieva; Ivy Tsz-Lo Wong; Sihan Wu; Siavash R Dehkordi; Connor V Duffy; Katerina Kraft; Jun Tang; Julia A Belk; John C Rose; M Ryan Corces; Jeffrey M Granja; Rui Li; Utkrisht Rajkumar; Jordan Friedlein; Anindya Bagchi; Ansuman T Satpathy; Robert Tjian; Stefan Mundlos; Vineet Bafna; Anton G Henssen; Paul S Mischel; Zhe Liu; Howard Y Chang
Journal:  Nature       Date:  2021-11-24       Impact factor: 69.504

4.  i-BLESS is an ultra-sensitive method for detection of DNA double-strand breaks.

Authors:  Anna Biernacka; Yingjie Zhu; Magdalena Skrzypczak; Romain Forey; Benjamin Pardo; Marta Grzelak; Jules Nde; Abhishek Mitra; Andrzej Kudlicki; Nicola Crosetto; Philippe Pasero; Maga Rowicka; Krzysztof Ginalski
Journal:  Commun Biol       Date:  2018-10-31
  4 in total

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