OBJECTIVE: Oxidative stress is involved in the pathogenesis of atherosclerosis. 8-Hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative product of DNA, is a sensitive biomarker to reflect oxidative stress status in vivo. However, the circulating 8-OHdG levels in patients with coronary artery disease (CAD) have not yet been elucidated. The purpose of this study is to investigate whether serum 8-OHdG levels are associated with the presence and severity of CAD. METHODS: We measured serum 8-OHdG levels by enzyme-linked immunosorbent assay in 127 patients with suspected CAD who underwent coronary angiography. The severity of CAD was assessed by the number of diseased vessels and Gensini score. RESULTS: The serum 8-OHdG levels in patients with CAD were significantly higher than those in patients with normal coronary arteries [median (interquartile range), 0.41 (0.30-0.57) ng/ml versus 0.32 (0.25-0.43) ng/ml, P = 0.001]. Log (8-OHdG) levels increased with the number of diseased vessels (P = 0.002) and significantly correlated with Gensini score (r = 0.379, P = 0.001). The multivariable logistic regression analysis showed that serum 8-OHdG was independently associated with the presence of CAD (odds ratio, 1.318; P = 0.027). CONCLUSION: In conclusion, the serum 8-OHdG levels are increased in patients with CAD and are associated with the severity of coronary artery stenosis. 8-OHdG might serve as an independent factor for predicting CAD.
OBJECTIVE: Oxidative stress is involved in the pathogenesis of atherosclerosis. 8-Hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative product of DNA, is a sensitive biomarker to reflect oxidative stress status in vivo. However, the circulating 8-OHdG levels in patients with coronary artery disease (CAD) have not yet been elucidated. The purpose of this study is to investigate whether serum 8-OHdG levels are associated with the presence and severity of CAD. METHODS: We measured serum 8-OHdG levels by enzyme-linked immunosorbent assay in 127 patients with suspected CAD who underwent coronary angiography. The severity of CAD was assessed by the number of diseased vessels and Gensini score. RESULTS: The serum 8-OHdG levels in patients with CAD were significantly higher than those in patients with normal coronary arteries [median (interquartile range), 0.41 (0.30-0.57) ng/ml versus 0.32 (0.25-0.43) ng/ml, P = 0.001]. Log (8-OHdG) levels increased with the number of diseased vessels (P = 0.002) and significantly correlated with Gensini score (r = 0.379, P = 0.001). The multivariable logistic regression analysis showed that serum 8-OHdG was independently associated with the presence of CAD (odds ratio, 1.318; P = 0.027). CONCLUSION: In conclusion, the serum 8-OHdG levels are increased in patients with CAD and are associated with the severity of coronary artery stenosis. 8-OHdG might serve as an independent factor for predicting CAD.
Authors: Camilo Gomez; Laisel Martinez; Annia Mesa; Juan C Duque; Luis A Escobar; Si M Pham; Roberto I Vazquez-Padron Journal: Biosci Rep Date: 2015-06-12 Impact factor: 3.840
Authors: Linda Turnu; Alessandro Di Minno; Benedetta Porro; Isabella Squellerio; Alice Bonomi; Chiara Maria Manega; José Pablo Werba; Alessandro Parolari; Elena Tremoli; Viviana Cavalca Journal: Oxid Med Cell Longev Date: 2017-06-04 Impact factor: 6.543
Authors: Seun Deuk Hwang; Yoon Ji Kim; Sang Heun Lee; Deok Kyu Cho; Yun Hyeong Cho; Sung Jin Moon; Sang Choel Lee; Soo Young Yoon Journal: Yonsei Med J Date: 2013-11 Impact factor: 2.759
Authors: Merlin C Thomas; Mark Woodward; Qiang Li; Raelene Pickering; Christos Tikellis; Neil Poulter; Mark E Cooper; Michel Marre; Sophia Zoungas; John Chalmers Journal: J Am Heart Assoc Date: 2018-06-30 Impact factor: 5.501