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Literature DB >> 21401111

LNA for optimization of fluorescent oligonucleotide probes: improved spectral properties and target binding.

Irina V Astakhova¹, Alexey V Ustinov, Vladimir A Korshun, Jesper Wengel.

Abstract

Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.

Entities: Chemical

Mesh：

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Year: 2011 PMID： 21401111 DOI： 10.1021/bc1005027

Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774

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Cited

12 in total

1. DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.

Authors: Akimitsu Okamoto
Journal: Adv Exp Med Biol Date: 2021 Impact factor: 2.622

2. Recognition of double-stranded DNA using energetically activated duplexes with interstrand zippers of 1-, 2- or 4-pyrenyl-functionalized O2'-alkylated RNA monomers.

Authors: Saswata Karmakar; Andreas S Madsen; Dale C Guenther; Bradley C Gibbons; Patrick J Hrdlicka
Journal: Org Biomol Chem Date: 2014-08-21 Impact factor: 3.876

3. Functionalized tricyclic cytosine analogues provide nucleoside fluorophores with improved photophysical properties and a range of solvent sensitivities.

Authors: Brittney J Rodgers; Nada A Elsharif; Nisha Vashisht; Macy M Mingus; Mark A Mulvahill; Gudrun Stengel; Robert D Kuchta; Byron W Purse
Journal: Chemistry Date: 2013-12-05 Impact factor: 5.236

4. Synthesis and characterization of oligodeoxyribonucleotides modified with 2'-thio-2'-deoxy-2'-S-(pyren-1-yl)methyluridine.

Authors: Brooke A Anderson; Patrick J Hrdlicka
Journal: Bioorg Med Chem Lett Date: 2015-07-07 Impact factor: 2.823

5. DNA strands with alternating incorporations of LNA and 2'-O-(pyren-1-yl)methyluridine: SNP-discriminating RNA detection probes.

Authors: Saswata Karmakar; Patrick J Hrdlicka
Journal: Chem Sci Date: 2013-09-01 Impact factor: 9.825

6. Identification and characterization of second-generation invader locked nucleic acids (LNAs) for mixed-sequence recognition of double-stranded DNA.

Authors: Sujay P Sau; Andreas S Madsen; Peter Podbevsek; Nicolai K Andersen; T Santhosh Kumar; Sanne Andersen; Rie L Rathje; Brooke A Anderson; Dale C Guenther; Saswata Karmakar; Pawan Kumar; Janez Plavec; Jesper Wengel; Patrick J Hrdlicka
Journal: J Org Chem Date: 2013-09-25 Impact factor: 4.354

LNA for optimization of fluorescent oligonucleotide probes: improved spectral properties and target binding.

1. DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.

2. Recognition of double-stranded DNA using energetically activated duplexes with interstrand zippers of 1-, 2- or 4-pyrenyl-functionalized O2'-alkylated RNA monomers.

3. Functionalized tricyclic cytosine analogues provide nucleoside fluorophores with improved photophysical properties and a range of solvent sensitivities.

4. Synthesis and characterization of oligodeoxyribonucleotides modified with 2'-thio-2'-deoxy-2'-S-(pyren-1-yl)methyluridine.

5. DNA strands with alternating incorporations of LNA and 2'-O-(pyren-1-yl)methyluridine: SNP-discriminating RNA detection probes.

6. Identification and characterization of second-generation invader locked nucleic acids (LNAs) for mixed-sequence recognition of double-stranded DNA.

7. Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA.

8. Pyrene-nucleobase conjugates: synthesis, oligonucleotide binding and confocal bioimaging studies.

Review 9. Fluorescent probes for nucleic Acid visualization in fixed and live cells.

Review 10. Molecular beacons of xeno-nucleic acid for detecting nucleic acid.