Literature DB >> 21400694

Next generation sequence assembly with AMOS.

Todd J Treangen1, Dan D Sommer, Florent E Angly, Sergey Koren, Mihai Pop.   

Abstract

A Modular Open-Source Assembler (AMOS) was designed to offer a modular approach to genome assembly. AMOS includes a wide range of tools for assembly, including the lightweight de novo assemblers Minimus and Minimo, and Bambus 2, a robust scaffolder able to handle metagenomic and polymorphic data. This protocol describes how to configure and use AMOS for the assembly of Next Generation sequence data. Additionally, we provide three tutorial examples that include bacterial, viral, and metagenomic datasets with specific tips for improving assembly quality.
© 2011 by John Wiley & Sons, Inc.

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Year:  2011        PMID： 21400694      PMCID： PMC3072823          DOI： 10.1002/0471250953.bi1108s33

Source DB:  PubMed          Journal:  Curr Protoc Bioinformatics        ISSN： 1934-3396


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