Literature DB >> 21400681

Measurement of type I interferon production.

Rosalind E Seeds1, Joanna L Miller.   

Abstract

The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays discussed. These assays therefore provide complementary methods to assess IFN production by murine cells.
© 2011 by John Wiley & Sons, Inc.

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Year:  2011        PMID： 21400681     DOI： 10.1002/0471142735.im1421s92

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  5 in total

1.  A panel of synthetic antibodies that selectively recognize and antagonize members of the interferon alpha family.

Authors:  S Miersch; S Kuruganti; M R Walter; S S Sidhu
Journal:  Protein Eng Des Sel       Date:  2017-09-01       Impact factor: 1.650

2.  ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells.

Authors:  Konrad Büssow; Philipp Themann; Sabine Luu; Paul Pentrowski; Claudia Harting; Mira Majewski; Veith Vollmer; Mario Köster; Martina Grashoff; Rainer Zawatzky; Joop Van den Heuvel; Andrea Kröger; Thomas Böldicke
Journal:  PLoS One       Date:  2019-04-16       Impact factor: 3.240

3.  IFNβ Is a Potent Adjuvant for Cancer Vaccination Strategies.

Authors:  Katherine M Audsley; Teagan Wagner; Clara Ta; Hannah V Newnes; Anthony C Buzzai; Samantha A Barnes; Ben Wylie; Jesse Armitage; Tsuneyasu Kaisho; Anthony Bosco; Alison McDonnell; Mark Cruickshank; Vanessa S Fear; Bree Foley; Jason Waithman
Journal:  Front Immunol       Date:  2021-09-06       Impact factor: 7.561

4.  The viral context instructs the redundancy of costimulatory pathways in driving CD8(+) T cell expansion.

Authors:  Suzanne P M Welten; Anke Redeker; Kees L M C Franken; Jennifer D Oduro; Ferry Ossendorp; Luka Čičin-Šain; Cornelis J M Melief; Peter Aichele; Ramon Arens
Journal:  Elife       Date:  2015-08-11       Impact factor: 8.140

5.  CD8+XCR1neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors.

Authors:  Ben Wylie; James Read; Anthony C Buzzai; Teagan Wagner; Niamh Troy; Genevieve Syn; Shane R Stone; Bree Foley; Anthony Bosco; Mark N Cruickshank; Jason Waithman
Journal:  Front Immunol       Date:  2019-01-16       Impact factor: 7.561
  5 in total

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