Literature DB >> 21400674

Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension.

Robin Treuer1, Shelley E Haydel.   

Abstract

Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.
© 2011 by John Wiley & Sons, Inc.

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Year:  2011        PMID: 21400674      PMCID: PMC3071241          DOI: 10.1002/9780471729259.mc10a06s20

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  7 in total

1.  A modified Ziehl-Neelsen stain for mycobacteria.

Authors:  J L Allen
Journal:  Med Lab Sci       Date:  1992-06

2.  Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli.

Authors:  N Selvakumar; Fathima Rahman; S Rajasekaran; P R Narayanan; Thomas R Frieden
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

3.  IMPROVED TECHNIQUE FOR THE DETECTION OF ACID-FAST BACILLIBY FLUORESCENCE.

Authors:  S W GILKERSON; O KANNER
Journal:  J Bacteriol       Date:  1963-10       Impact factor: 3.490

4.  A NOTE ON UHLENHUTHS METHOD FOR SPUTUM EXAMINATION, FOR TUBERCLE BACILLI.

Authors:  J J Kinyoun
Journal:  Am J Public Health (N Y)       Date:  1915-09

5.  An essential two-component signal transduction system in Mycobacterium tuberculosis.

Authors:  T C Zahrt; V Deretic
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

6.  Specific detection and quantification of culturable and non-culturable mycobacteria in metalworking fluids by fluorescence-based methods.

Authors:  S B Selvaraju; I U H Khan; J S Yadav
Journal:  Lett Appl Microbiol       Date:  2008-09-16       Impact factor: 2.858

7.  Laboratory maintenance of Mycobacterium marinum.

Authors:  Lian-Yong Gao; Joanna Manoranjan
Journal:  Curr Protoc Microbiol       Date:  2005-07
  7 in total
  1 in total

Review 1.  An overview of calf diarrhea - infectious etiology, diagnosis, and intervention.

Authors:  Yong-Il Cho; Kyoung-Jin Yoon
Journal:  J Vet Sci       Date:  2013-12-27       Impact factor: 1.672

  1 in total

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