OBJECTIVE: The aim of this study was to understand the role of cyclin-dependent kinase-associated protein phosphatase (KAP) in renal cancer cell growth. MATERIALS AND METHODS: Renal cell carcinoma (RCC) tissues from 58 patients receiving surgical resection were included for immunohistochemistry analysis. Additionally, human embryonic kidney (HEK293) cells overexpressing KAP were established for tumorigenicity experiments. RESULTS: Clinicopathologic analysis indicated that poorly differentiated RCCs with a higher histological grade (grade 3/4) were associated with a higher proportion of KAP-positive cells (P < 0.001) as well as cytoplasmic expression of KAP (P < 0.05). HEK293 cells overexpressing KAP had a higher growth rate, greater resistance to TNF-α mediated increment of caspase 3 activity, a shorter cell cycle time, and greater ability of cell invasion. Tumorigenicity experiments showed that KAP-overexpressing cells generated significantly larger xenograft tumors in nude mice compared with mock controls (P = 0.032). CONCLUSIONS: KAP expression was associated with poorly differentiated RCCs and overexpression of KAP in renal cells enhanced cell proliferation, resistance to apoptosis, invasive ability, and xenograft tumor formation.
OBJECTIVE: The aim of this study was to understand the role of cyclin-dependent kinase-associated protein phosphatase (KAP) in renal cancer cell growth. MATERIALS AND METHODS:Renal cell carcinoma (RCC) tissues from 58 patients receiving surgical resection were included for immunohistochemistry analysis. Additionally, humanembryonic kidney (HEK293) cells overexpressing KAP were established for tumorigenicity experiments. RESULTS: Clinicopathologic analysis indicated that poorly differentiated RCCs with a higher histological grade (grade 3/4) were associated with a higher proportion of KAP-positive cells (P < 0.001) as well as cytoplasmic expression of KAP (P < 0.05). HEK293 cells overexpressing KAP had a higher growth rate, greater resistance to TNF-α mediated increment of caspase 3 activity, a shorter cell cycle time, and greater ability of cell invasion. Tumorigenicity experiments showed that KAP-overexpressing cells generated significantly larger xenograft tumors in nude mice compared with mock controls (P = 0.032). CONCLUSIONS:KAP expression was associated with poorly differentiated RCCs and overexpression of KAP in renal cells enhanced cell proliferation, resistance to apoptosis, invasive ability, and xenograft tumor formation.
Authors: Ayanda M Magwenyane; Samuel C Ugbaja; Daniel G Amoako; Anou M Somboro; Rene B Khan; Hezekiel M Kumalo Journal: Comput Math Methods Med Date: 2022-05-31 Impact factor: 2.809
Authors: Eira Valeria Barrón; Edgar Roman-Bassaure; Ana Laura Sánchez-Sandoval; Ana María Espinosa; Mariano Guardado-Estrada; Ingrid Medina; Eligia Juárez; Ana Alfaro; Miriam Bermúdez; Rubén Zamora; Carlos García-Ruiz; Juan Carlos Gomora; Susana Kofman; E Martha Pérez-Armendariz; Jaime Berumen Journal: PLoS One Date: 2015-09-15 Impact factor: 3.240