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Literature DB >> 2139652

An upstream uncD sequence modulates translation of Escherichia coli uncC.

Abstract

The effect of upstream uncD sequences on expression of the Escherichia coli uncC gene, encoding the epsilon subunit of F1-ATPase, was studied. uncC expression was reduced severalfold in plasmid constructs bearing, in addition to uncC, a region of uncD located between 85 and 119 bases upstream from the uncC initiation codon. This reduction was independent of in-frame translation of the uncD sequences. An mRNA stem-loop structure in which sequences located within the inhibitory region of uncD base pair with the uncDC intercistronic region is suggested to function in modulating uncC expression.

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Year: 1990 PMID： 2139652 PMCID： PMC208927 DOI： 10.1128/jb.172.5.2782-2784.1990

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

An upstream uncD sequence modulates translation of Escherichia coli uncC.

1. Beta-lactamase assays.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Determinants of translational initiation efficiency in the atp operon of Escherichia coli.

4. Altered translation of the uncC gene coding for the epsilon subunit of the F1F0-ATPase of Escherichia coli.

Review 5. The unc operon. Nucleotide sequence, regulation and structure of ATP-synthase.

6. Cloning and expression of uncI, the first gene of the unc operon of Escherichia coli.

7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

8. Stoichiometry of subunits in the H+-ATPase complex of Escherichia coli.

9. A system for shotgun DNA sequencing.

10. Regulation of ribosomal RNA promoters with a synthetic lac operator.

1. RNase E-dependent cleavages in the 5' and 3' regions of the Escherichia coli unc mRNA.

2. Translation through an uncDC mRNA secondary structure governs the level of uncC expression in Escherichia coli.