| Literature DB >> 21396180 |
Yu Tian1, Michelle M Martinez, Dimitri Pappas.
Abstract
Over the years fluorescence correlation spectroscopy (FCS) has proven to be a useful technique that has been utilized in several fields of study. Although FCS initially suffered from poor signal-to-noise ratios, the incorporation of confocal microscopy has overcome this drawback and transformed FCS into a sensitive technique with high figures of merit. In addition, tandem methods have evolved to include dual-color cross-correlation, total internal reflection fluorescence correlation, and fluorescence lifetime correlation spectroscopy combined with time-correlated single-photon counting. In this review, we discuss several applications of FSC for biochemical, microfluidic, and cellular investigations.Entities:
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Year: 2011 PMID: 21396180 PMCID: PMC3071976 DOI: 10.1366/10-06224
Source DB: PubMed Journal: Appl Spectrosc ISSN: 0003-7028 Impact factor: 2.388