Literature DB >> 21393857

Influence of temperature during crystallization setup on precipitate formation and crystal shape of a metalloendopeptidase.

Xenia Bogdanović1, Winfried Hinrichs.   

Abstract

It is well known that protein crystallization is affected by several different parameters such as the composition of the reservoir solution, the protein concentration, the pH and the temperature. An effect of different temperatures during setup of crystallization experiments was observed for a metalloendopeptidase (AsaP1(E294A)). Spontaneous protein precipitation was reduced and the crystal shape could be improved by decreasing the temperature during crystallization setup.

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Year:  2011        PMID： 21393857      PMCID： PMC3053177          DOI： 10.1107/S1744309111001783

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  6 in total

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-10

Review 2.  Seeds to crystals.

Authors:  Terese Bergfors
Journal:  J Struct Biol       Date:  2003-04       Impact factor: 2.867

3.  Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from Aeromonas salmonicida subsp. achromogenes.

Authors:  Xenia Bogdanović; Rajesh Kumar Singh; Johanna Hentschke; Bjarnheidur K Gudmundsdóttir; Winfried Hinrichs
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-06-27

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Journal:  Eur J Biochem       Date:  1990-04-20

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Authors:  D M Blow; N E Chayen; L F Lloyd; E Saridakis
Journal:  Protein Sci       Date:  1994-10       Impact factor: 6.725

6.  The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor.

Authors:  Helga Arnadottir; Iris Hvanndal; Valgerdur Andresdottir; Sarah E Burr; Joachim Frey; Bjarnheidur K Gudmundsdottir
Journal:  J Bacteriol       Date:  2008-10-24       Impact factor: 3.490
  6 in total
  1 in total

1.  Purification, crystallization and preliminary crystallographic analysis of banyan peroxidase.

Authors:  Anurag Sharma; Gottfried J Palm; Moni Kumari; Santosh Panjikar; M V Jagannadham; Winfried Hinrichs
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-07-31
  1 in total

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