Literature DB >> 21393846

Isolation, purification, crystallization and preliminary X-ray studies of two 30 kDa proteins from silkworm haemolymph.

Agnieszka J Pietrzyk1, Anna Bujacz, Małgorzata Łochyńska, Mariusz Jaskólski, Grzegorz Bujacz.   

Abstract

Juvenile hormone-binding protein (JHBP) and the low-molecular-mass lipoprotein PBMHP-12 belong to a group of 30 kDa proteins that comprise the major protein component of the haemolymph specific to the fifth-instar larvae stage of the mulberry silkworm Bombyx mori L. Proteins from this group are often essential for the development of the insect. In a project aimed at crystallographic characterization of B. mori JHBP (BmJHBP), it was copurified together with PBMHP-12. Eventually, the two proteins were isolated and crystallized separately. The BmJHBP crystals were orthorhombic (space group C222(1)) and the PBMHP-12 crystals were triclinic. The crystals diffracted X-rays to 2.9 Å (BmJHBP) and 1.3 Å (PBMHP-12) resolution.

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Year:  2011        PMID： 21393846      PMCID： PMC3053166          DOI： 10.1107/S1744309110054564

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  20 in total

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Authors:  Robert Kołodziejczyk; Marian Kochman; Grzegorz Bujacz; Piotr Dobryszycki; Andrzej Ozyhar; Mariusz Jaskolski
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-02-21

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Authors:  K H Trautmann
Journal:  Z Naturforsch B       Date:  1972-03       Impact factor: 1.047

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Journal:  Biochim Biophys Acta       Date:  1988-02-28

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8.  The juvenile hormone binding protein of silkworm haemolymph: gene and functional analysis.

Authors:  A M Vermunt; M Kamimura; M Hirai; M Kiuchi; T Shiotsuki
Journal:  Insect Mol Biol       Date:  2001-04       Impact factor: 3.585

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Journal:  Infect Immun       Date:  1988-11       Impact factor: 3.441

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Authors:  Janusz Debski; Aleksandra Wysłouch-Cieszyńska; Michał Dadlez; Krystyna Grzelak; Barbara Kłudkiewicz; Robert Kołodziejczyk; Anna Lalik; Andrzej Ozyhar; Marian Kochman
Journal:  Arch Biochem Biophys       Date:  2004-01-15       Impact factor: 4.013
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  3 in total

1.  A proteomic view on the developmental transfer of homologous 30 kDa lipoproteins from peripheral fat body to perivisceral fat body via hemolymph in silkworm, Bombyx mori.

Authors:  Britto Cathrin Pakkianathan; Nitin Kumar Singh; Muthukalingan Krishnan; Simone König
Journal:  BMC Biochem       Date:  2012-02-28       Impact factor: 4.059

2.  Crystal structure of Bombyx mori lipoprotein 6: comparative structural analysis of the 30-kDa lipoprotein family.

Authors:  Agnieszka J Pietrzyk; Anna Bujacz; Malgorzata Łochynska; Mariusz Jaskolski; Grzegorz Bujacz
Journal:  PLoS One       Date:  2014-11-07       Impact factor: 3.240

3.  Two crystal structures of Bombyx mori lipoprotein 3 - structural characterization of a new 30-kDa lipoprotein family member.

Authors:  Agnieszka J Pietrzyk; Anna Bujacz; Jochen Mueller-Dieckmann; Malgorzata Lochynska; Mariusz Jaskolski; Grzegorz Bujacz
Journal:  PLoS One       Date:  2013-04-16       Impact factor: 3.240
  3 in total

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