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Literature DB >> 21393835

Purification, crystallization and preliminary crystallographic analysis of the CBS-domain protein MJ1004 from Methanocaldococcus jannaschii.

Iker Oyenarte¹, María Lucas, Inmaculada Gómez García, Luis Alfonso Martínez-Cruz.

Abstract

The purification and preliminary crystallographic analysis of the archaeal CBS-domain protein MJ1004 from Methanocaldococcus jannaschii are described. The native protein was overexpressed, purified and crystallized in the monoclinic space group P2(1), with unit-cell parameters a=54.4, b=53.8, c=82.6 Å, β=106.1°. The crystals diffracted X-rays to 2.7 Å resolution using synchrotron radiation. Matthews-volume calculations suggested the presence of two molecules in the asymmetric unit that are likely to correspond to a dimeric species, which is also observed in solution.

Entities: Species

Mesh：

Substances：
Archaeal Proteins

Year: 2011 PMID： 21393835 PMCID： PMC3053155 DOI： 10.1107/S1744309110053479

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

40 in total

Review 1. Messenger RNA stability and its role in control of gene expression in bacteria and phages.

Authors: M Grunberg-Manago
Journal: Annu Rev Genet Date: 1999 Impact factor: 16.830

2. A role for CBS domain 2 in trafficking of chloride channel CLC-5.

Authors: Georgina Carr; Nicholas Simmons; John Sayer
Journal: Biochem Biophys Res Commun Date: 2003-10-17 Impact factor: 3.575

3. Crystal structure of a tandem cystathionine-beta-synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 A resolution.

Authors: Mitchell D Miller; Robert Schwarzenbacher; Frank von Delft; Polat Abdubek; Eileen Ambing; Tanya Biorac; Linda S Brinen; Jaume M Canaves; Jamison Cambell; Hsiu-Ju Chiu; Xiaoping Dai; Ashley M Deacon; Mike DiDonato; Marc-André Elsliger; Said Eshagi; Ross Floyd; Adam Godzik; Carina Grittini; Slawomir K Grzechnik; Eric Hampton; Lukasz Jaroszewski; Cathy Karlak; Heath E Klock; Eric Koesema; John S Kovarik; Andreas Kreusch; Peter Kuhn; Scott A Lesley; Inna Levin; Daniel McMullan; Timothy M McPhillips; Andrew Morse; Kin Moy; Jie Ouyang; Rebecca Page; Kevin Quijano; Alyssa Robb; Glen Spraggon; Raymond C Stevens; Henry van den Bedem; Jeff Velasquez; Juli Vincent; Xianhong Wang; Bill West; Guenter Wolf; Qingping Xu; Keith O Hodgson; John Wooley; Ian A Wilson
Journal: Proteins Date: 2004-10-01

4. Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100.

Authors: María Lucas; Danel Kortazar; Egoitz Astigarraga; José A Fernández; Jose M Mato; María Luz Martínez-Chantar; Luis Alfonso Martínez-Cruz
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2008-09-30

5. Purification, crystallization and preliminary crystallographic analysis of protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homologue of gamma-AMPK.

Authors: Inmaculada Gómez García; Danel Kortázar; Iker Oyenarte; José María Mato; María Luz Martínez-Chantar; Luis Alfonso Martínez-Cruz
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2009-07-30

6. Binding of S-methyl-5'-thioadenosine and S-adenosyl-L-methionine to protein MJ0100 triggers an open-to-closed conformational change in its CBS motif pair.

Authors: María Lucas; José Antonio Encinar; Egoitz Astigarraga Arribas; Iker Oyenarte; Inmaculada Gómez García; Danel Kortazar; José A Fernández; Jose M Mato; María Luz Martínez-Chantar; Luis Alfonso Martínez-Cruz
Journal: J Mol Biol Date: 2009-12-21 Impact factor: 5.469

7. Solvent content of protein crystals.

Authors: B W Matthews
Journal: J Mol Biol Date: 1968-04-28 Impact factor: 5.469

8. The CBS domain protein MJ0729 of Methanocaldococcus jannaschii is a thermostable protein with a pH-dependent self-oligomerization.

Authors: Luis Alfonso Martínez-Cruz; José A Encinar; Danel Kortazar; Jesús Prieto; Javier Gómez; Pablo Fernández-Millán; María Lucas; Egoitz Astigarraga Arribas; José Andrés Fernández; María Luz Martínez-Chantar; José M Mato; José Luis Neira
Journal: Biochemistry Date: 2009-03-31 Impact factor: 3.162

Purification, crystallization and preliminary crystallographic analysis of the CBS-domain protein MJ1004 from Methanocaldococcus jannaschii.

Review 1. Messenger RNA stability and its role in control of gene expression in bacteria and phages.

2. A role for CBS domain 2 in trafficking of chloride channel CLC-5.

3. Crystal structure of a tandem cystathionine-beta-synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 A resolution.

4. Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100.

5. Purification, crystallization and preliminary crystallographic analysis of protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homologue of gamma-AMPK.

6. Binding of S-methyl-5'-thioadenosine and S-adenosyl-L-methionine to protein MJ0100 triggers an open-to-closed conformational change in its CBS motif pair.

7. Solvent content of protein crystals.

8. The CBS domain protein MJ0729 of Methanocaldococcus jannaschii is a thermostable protein with a pH-dependent self-oligomerization.

9. Mg2+-sensing mechanism of Mg2+ transporter MgtE probed by molecular dynamics study.

Review 10. Scaling and assessment of data quality.

1. Improving the chances of successful protein structure determination with a random forest classifier.

Review 2. The "Sticky Patch" Model of Crystallization and Modification of Proteins for Enhanced Crystallizability.

3. Crystals on the cover 2012.