| Literature DB >> 21392978 |
Waseem Raza1, Kousar Makeen, Yang Wang, Yangchun Xu, Shen Qirong.
Abstract
The optimization, purification and characterization of an extracellular polysaccharide (EPS) from a bacterium Paenibacillus polymyxa SQR-21 (SQR-21) were investigated. The results showed that SQR-21 produced one kind of EPS having molecular weight of 8.96 × 10(5)Da. The EPS was comprised of mannose, galactose and glucose in a ratio of 1.23:1.14:1. The ratio of monosaccharides and glucuronic acid was 7.5:1. The preferable culture conditions for EPS production were pH 6.5, temperature 30°C for 96 h with yeast extract and galactose as best N and C sources, respectively. The maximum EPS production (3.44 g L(-1)) was achieved with galactose 48.5 g L(-1), Fe(3+) 242 μM and Ca(2+) 441 μM. In addition, the EPS showed good superoxide scavenging, flocculating and metal chelating activities while moderate inhibition of lipid peroxidation and reducing activities were determined. These results showed the great potential of EPS produced by SQR-21 to be used in industry in place of synthetic compounds.Entities:
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Year: 2011 PMID： 21392978 DOI： 10.1016/j.biortech.2011.02.033
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642