Literature DB >> 21391196

[Diagnosis of protozoa by loop-mediated isothermal amplification: (LAMP)].

Zeynep Kolören1, Cumhur Avşar, Zülal Atlı Şekeroğlu.   

Abstract

Plasmodium, Isospora, Toxoplasma, Babesia and Cryptosporidium are parasites which have a significant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan. Classical methods are not adequate for the identification of some protozoon; also, molecular methods have to be used in designating the distinctions between the species. In recent years, one of the molecular methods which has been used frequently in the identification of protozoon is the technique of LAMP. With the aid of the LAMP technique, from which it is possible to obtain reliable outcomes without the contribution of technical skills and professional equipment, in constant temperature it is possible to have a great number of copies from the targeted DNA in a short period. The aim of this collation is to give information about the usage of the technique of LAMP in the identification of the protozoa which are important for human health and the comparison between the technique of LAMP and other molecular methods.

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Year:  2010        PMID: 21391196     DOI: 10.5152/tpd.2010.16

Source DB:  PubMed          Journal:  Turkiye Parazitol Derg        ISSN: 1300-6320


  1 in total

1.  Development of a rapid, simple method for detecting Naegleria fowleri visually in water samples by loop-mediated isothermal amplification (LAMP).

Authors:  Aongart Mahittikorn; Hirotake Mori; Supaluk Popruk; Amonrattana Roobthaisong; Chantira Sutthikornchai; Khuanchai Koompapong; Sukhontha Siri; Yaowalark Sukthana; Duangporn Nacapunchai
Journal:  PLoS One       Date:  2015-03-30       Impact factor: 3.240

  1 in total

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