| Literature DB >> 21391046 |
Munehide Nakatsugawa1, Yoshihiko Hirohashi, Toshihiko Torigoe, Satoko Inoda, Kenji Kiriyama, Yasuaki Tamura, Eiji Sato, Hiroki Takahashi, Noriyuki Sato.
Abstract
Human leukocyte antigen (HLA) typing is essential to carry out HLA-class I restricted antigenic peptide-based cancer immunotherapy. To establish a one-step polymerase chain reaction-sequence-specific primer (PCR-SSP) method, we designed two novel HLA-A24-specific primer sets and determined the optimal conditions for specific amplification. Then, we performed HLA-A24 typing of two healthy donors' and 17 cancer patients' peripheral blood with serological typing and PCR-SSP typing. Eleven of the 19 cases were determined HLA-A24-positive by the PCR-SSP method precisely; however, five cases showed false positive with serological analysis. Thus, for HLA-A24 typing in the Japanese population, the PCR-SSP method is faster and more accurate than serological typing.Entities:
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Year: 2011 PMID： 21391046 DOI： 10.1080/15321819.2010.543219
Source DB: PubMed Journal: J Immunoassay Immunochem ISSN： 1532-1819