Literature DB >> 21387205

Generation and characterization of koi herpesvirus recombinants lacking viral enzymes of nucleotide metabolism.

Walter Fuchs1, Dieter Fichtner, Sven M Bergmann, Thomas C Mettenleiter.   

Abstract

Koi herpesvirus (KHV) causes a fatal disease in koi and common carp, but no reliable and genetically characterized vaccines are available up to now. Therefore, we generated KHV recombinants possessing deletions within the viral ribonucleotide reductase (RNR), thymidine kinase (TK), dUTPase, or TK and dUTPase genes, and their corresponding rescuants. All KHV mutants were replication competent in cultured cells. Whereas plaque sizes and titers of RNR-negative KHV were reduced, replication of the other mutants was not affected. Experimental infection of carp indicated attenuation of TK- or dUTPase-deleted KHV, and PCR analysis of tissue samples permitted differentiation of mutant from wild-type virus.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21387205     DOI： 10.1007/s00705-011-0953-8

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  10 in total

1.  Koi herpes virus: a review and risk assessment of Indian aquaculture.

Authors:  Gaurav Rathore; Gokhlesh Kumar; T Raja Swaminathan; P Swain
Journal:  Indian J Virol       Date:  2012-09-06

2.  Inoculation of cyprinid herpesvirus 3 (CyHV-3) on common carp brain cells-influence of process parameters on virus yield.

Authors:  A Mletzko; A Amtmann; S Bergmann; P Lee; J Christian; R Buchholz; A Becker
Journal:  In Vitro Cell Dev Biol Anim       Date:  2017-06-27       Impact factor: 2.416

Review 3.  CyHV-3: the third cyprinid herpesvirus.

Authors:  Michael Gotesman; Julia Kattlun; Sven M Bergmann; Mansour El-Matbouli
Journal:  Dis Aquat Organ       Date:  2013-07-22       Impact factor: 1.802

4.  Rational development of an attenuated recombinant cyprinid herpesvirus 3 vaccine using prokaryotic mutagenesis and in vivo bioluminescent imaging.

Authors:  Maxime Boutier; Maygane Ronsmans; Ping Ouyang; Guillaume Fournier; Anca Reschner; Krzysztof Rakus; Gavin S Wilkie; Frédéric Farnir; Calixte Bayrou; François Lieffrig; Hong Li; Daniel Desmecht; Andrew J Davison; Alain Vanderplasschen
Journal:  PLoS Pathog       Date:  2015-02-20       Impact factor: 6.823

Review 5.  Cyprinid herpesvirus 3: an interesting virus for applied and fundamental research.

Authors:  Krzysztof Rakus; Ping Ouyang; Maxime Boutier; Maygane Ronsmans; Anca Reschner; Catherine Vancsok; Joanna Jazowiecka-Rakus; Alain Vanderplasschen
Journal:  Vet Res       Date:  2013-09-27       Impact factor: 3.683

6.  Identification of an essential virulence gene of cyprinid herpesvirus 3.

Authors:  Maxime Boutier; Yuan Gao; Catherine Vancsok; Nicolás M Suárez; Andrew J Davison; Alain Vanderplasschen
Journal:  Antiviral Res       Date:  2017-07-06       Impact factor: 5.970

Review 7.  A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches.

Authors:  Jie Ma; Timothy J Bruce; Evan M Jones; Kenneth D Cain
Journal:  Microorganisms       Date:  2019-11-16

8.  In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi.

Authors:  Michael Gotesman; Hatem Soliman; Robert Besch; Mansour El-Matbouli
Journal:  J Virol Methods       Date:  2014-06-02       Impact factor: 2.014

9.  Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence.

Authors:  Sean J Monaghan; Kim D Thompson; James E Bron; Sven M Bergmann; Tae S Jung; Takashi Aoki; K Fiona Muir; Malte Dauber; Sven Reiche; Diana Chee; Shin M Chong; Jing Chen; Alexandra Adams
Journal:  Vet Res       Date:  2016-01-08       Impact factor: 3.683

10.  Antibody screening identifies 78 putative host proteins involved in Cyprinid herpesvirus 3 infection or propagation in common carp, Cyprinus carpio L.

Authors:  M Gotesman; H Soliman; M El-Matbouli
Journal:  J Fish Dis       Date:  2013-01-24       Impact factor: 2.767
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.