Literature DB >> 2138562

Purification and characterization of a 26-kDa competitive inhibitor of interleukin 1.

G J Mazzei1, P L Seckinger, J M Dayer, A R Shaw.   

Abstract

The urine of patients with fever above 39 degrees C contains an inhibitor of interleukin 1. Herein we describe the purification of the interleukin 1 inhibitor by ion-exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26,000; its size is reduced to 24,000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 x 10(7) to 4.5 x 10(7) units/mg as tested on the human astrocytoma (U-373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor-binding assay.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2138562     DOI： 10.1002/eji.1830200332

Source DB:  PubMed          Journal:  Eur J Immunol        ISSN： 0014-2980            Impact factor:   5.532


  11 in total

Review 1.  Cytokines, receptors, and inhibitors.

Authors:  G Gehr; T Braun; W Lesslauer
Journal:  Clin Investig       Date:  1992-01

2.  Selective and early increase of IL-1 inhibitors, IL-6 and cortisol after elective surgery.

Authors:  F Di Padova; C Pozzi; M J Tondre; R Tritapepe
Journal:  Clin Exp Immunol       Date:  1991-07       Impact factor: 4.330

Review 3.  Cytokines. 2. Cytokines and lung inflammation: mechanisms of neutrophil recruitment to the lung.

Authors:  R M Strieter; N W Lukacs; T J Standiford; S L Kunkel
Journal:  Thorax       Date:  1993-07       Impact factor: 9.139

4.  Psoriatic skin reveals the in vivo presence of an epidermal IL-1 inhibitor.

Authors:  N I Kim; K D Cooper; G J Fisher; O Baadsgaard; J J Voorhees; C Hammerberg
Journal:  Arch Dermatol Res       Date:  1992       Impact factor: 3.017

5.  Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running.

Authors:  K Ostrowski; T Rohde; M Zacho; S Asp; B K Pedersen
Journal:  J Physiol       Date:  1998-05-01       Impact factor: 5.182

6.  Characterization of IL-1 inhibitory factor released from human alveolar macrophages as IL-1 receptor antagonist.

Authors:  M Takeuchi; S Nagai; H Nakada; H Aung; N Satake; H Kaneshima; T Izumi
Journal:  Clin Exp Immunol       Date:  1992-04       Impact factor: 4.330

7.  Flare-up of experimental arthritis in mice with murine recombinant IL-1.

Authors:  A A van de Loo; O J Arntz; W B van den Berg
Journal:  Clin Exp Immunol       Date:  1992-02       Impact factor: 4.330

8.  Conversion of the interleukin 1 receptor antagonist into an agonist by site-specific mutagenesis.

Authors:  G Ju; E Labriola-Tompkins; C A Campen; W R Benjamin; J Karas; J Plocinski; D Biondi; K L Kaffka; P L Kilian; S P Eisenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

Review 9.  Interleukin 1 receptor antagonist. A new member of the interleukin 1 family.

Authors:  W P Arend
Journal:  J Clin Invest       Date:  1991-11       Impact factor: 14.808

10.  Competition of IL-1 and IL-1ra determines lymphocyte response to delayed stimulation with PHA.

Authors:  M P Dabrowski; W Stankiewicz; T Płusa; A Chciałowski; S Szmigielski
Journal:  Mediators Inflamm       Date:  2001-06       Impact factor: 4.711
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.