Literature DB >> 21383139

Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation.

Jie Fu1, James B Munro, Scott C Blanchard, Joachim Frank.   

Abstract

mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.

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Year:  2011        PMID： 21383139      PMCID： PMC3064370          DOI： 10.1073/pnas.1101503108

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2009-12-17       Impact factor: 11.205

4.  Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation.

Authors:  Jingyi Fei; Jonathan E Bronson; Jake M Hofman; Rathi L Srinivas; Chris H Wiggins; Ruben L Gonzalez
Journal:  Proc Natl Acad Sci U S A       Date:  2009-08-27       Impact factor: 11.205

5.  Structures of the ribosome in intermediate states of ratcheting.

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Journal:  Science       Date:  2009-08-21       Impact factor: 47.728

Review 6.  Single-molecule observations of ribosome function.

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Journal:  Nat Struct Mol Biol       Date:  2009-04-12       Impact factor: 15.369

8.  Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome.

Authors:  Xabier Agirrezabala; Jianlin Lei; Julie L Brunelle; Rodrigo F Ortiz-Meoz; Rachel Green; Joachim Frank
Journal:  Mol Cell       Date:  2008-10-24       Impact factor: 17.970

9.  Particle-verification for single-particle, reference-based reconstruction using multivariate data analysis and classification.

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10.  A unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis.

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  19 in total

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3.  Structural characterization of mRNA-tRNA translocation intermediates.

Authors:  Xabier Agirrezabala; Hstau Y Liao; Eduard Schreiner; Jie Fu; Rodrigo F Ortiz-Meoz; Klaus Schulten; Rachel Green; Joachim Frank
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-30       Impact factor: 11.205

Review 4.  The translation elongation cycle-capturing multiple states by cryo-electron microscopy.

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Review 7.  Mechanistic insights into antibiotic action on the ribosome through single-molecule fluorescence imaging.

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Review 8.  Crystal structure of eukaryotic ribosome and its complexes with inhibitors.

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9.  Structure and mechanical properties of the ribosomal L1 stalk three-way junction.

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Review 10.  Intermediate states during mRNA-tRNA translocation.

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Journal:  Curr Opin Struct Biol       Date:  2012-08-17       Impact factor: 6.809
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