OBJECTIVE: Neural tube defects in diabetic embryopathy are associated with increased protein kinase C (PKC)β2 activity and programmed cell death (apoptosis). The apoptosis is triggered by caspase 8, which activates members of the Bcl-2 and caspase families, such as Bid and caspase 3. Whether PKCβ2 regulates caspase 8-induced apoptosis remains to be addressed. STUDY DESIGN: Mouse embryos at embryonic day 8.5 were cultured in a high concentration of glucose (22 mmol/L) and treated with PKCβ2 inhibitor (50 nmol/L) for 48 hours. The levels of apoptosis and activation of apoptotic factors were quantified using the terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling and Western blot assays, respectively. RESULTS: Reduction in the rate of neural tube defect by PKCβ2 inhibition is associated with significant decreases in the levels of apoptosis, and caspase 8, caspase 3, and Bid activation, and cytochrome C release from mitochondria, to the similar levels as in euglycemic controls (8.3 mmol/L; P < .05). CONCLUSION: PKCβ2 influences a caspase 8-regulated apoptotic pathway in diabetic embryopathy.
OBJECTIVE:Neural tube defects in diabetic embryopathy are associated with increased protein kinase C (PKC)β2 activity and programmed cell death (apoptosis). The apoptosis is triggered by caspase 8, which activates members of the Bcl-2 and caspase families, such as Bid and caspase 3. Whether PKCβ2 regulates caspase 8-induced apoptosis remains to be addressed. STUDY DESIGN:Mouse embryos at embryonic day 8.5 were cultured in a high concentration of glucose (22 mmol/L) and treated with PKCβ2 inhibitor (50 nmol/L) for 48 hours. The levels of apoptosis and activation of apoptotic factors were quantified using the terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling and Western blot assays, respectively. RESULTS: Reduction in the rate of neural tube defect by PKCβ2 inhibition is associated with significant decreases in the levels of apoptosis, and caspase 8, caspase 3, and Bid activation, and cytochrome C release from mitochondria, to the similar levels as in euglycemic controls (8.3 mmol/L; P < .05). CONCLUSION:PKCβ2 influences a caspase 8-regulated apoptotic pathway in diabetic embryopathy.
Authors: Jonathan P Godbout; James Pesavento; Matthew E Hartman; Scott R Manson; Gregory G Freund Journal: J Biol Chem Date: 2001-11-13 Impact factor: 5.157