Literature DB >> 2137337

Primary structure, in vitro expression and import into mitochondria of a 29/21-kDa subunit of complex I from Neurospora crassa.

A Videira1, M Tropschug, S Werner.   

Abstract

A full-length cDNA clone coding for a cytoplasmically-synthesized subunit of complex I from Neurospora crassa (apparent molecular mass of 29 kDa) was isolated. DNA sequencing revealed an open reading frame coding for a protein containing 201 amino acids. A molecular mass of 21323 Da was calculated. The precursor polypeptide was efficiently expressed in vitro and imported into isolated mitochondria. It is synthesized without a cleavable signal sequence and needs a membrane potential in order to bind to the mitochondrial membranes.

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Year:  1990        PMID： 2137337     DOI： 10.1016/0006-291x(90)91942-l

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

Review 1.  Biogenesis of respiratory complex I.

Authors:  U Schulte
Journal:  J Bioenerg Biomembr       Date:  2001-06       Impact factor: 2.945

2.  Primary structure and mitochondrial import in vitro of the 20.9 kDa subunit of complex I from Neurospora crassa.

Authors:  J E Azevedo; U Nehls; C Eckerskorn; H Heinrich; H Rothe; H Weiss; S Werner
Journal:  Biochem J       Date:  1992-11-15       Impact factor: 3.857

3.  The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein.

Authors:  D Preis; J C van der Pas; U Nehls; D A Röhlen; U Sackmann; U Jahnke; H Weiss
Journal:  Curr Genet       Date:  1990-07       Impact factor: 3.886

Review 4.  Exploiting mitochondria as targets for the development of new antifungals.

Authors:  Dongmei Li; Richard Calderone
Journal:  Virulence       Date:  2016-05-18       Impact factor: 5.882

5.  In organello assembly of respiratory-chain complex I: primary structure of the 14.8 kDa subunit of Neurospora crassa complex I.

Authors:  J E Azevedo; C Eckerskorn; S Werner
Journal:  Biochem J       Date:  1994-04-01       Impact factor: 3.857

6.  Cloning, in vitro mitochondrial import and membrane assembly of the 17.8 kDa subunit of complex I from Neurospora crassa.

Authors:  J E Azevedo; J Abrolat-Scharff; C Eckerskorn; S Werner
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

7.  Fungal-specific subunits of the Candida albicans mitochondrial complex I drive diverse cell functions including cell wall synthesis.

Authors:  Xiaodong She; Kasra Khamooshi; Yin Gao; Yongnian Shen; Yuxia Lv; Richard Calderone; William Fonzi; Weida Liu; Dongmei Li
Journal:  Cell Microbiol       Date:  2015-04-16       Impact factor: 3.715

8.  Inactivation of genes encoding subunits of the peripheral and membrane arms of neurospora mitochondrial complex I and effects on enzyme assembly.

Authors:  M Duarte; R Sousa; A Videira
Journal:  Genetics       Date:  1995-03       Impact factor: 4.562

9.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-07-25       Impact factor: 16.971
  9 in total

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