AIMS: This study aimed to identify relevant keratin subtypes that may associate with the pathogenesis of oral epithelial neoplasms. METHODS AND RESULTS: Expression of all the keratin subtypes was examined by cDNA microarray analysis of 43 oral squamous cell carcinoma (OSCC) cases. Immunohistochemical expression of the major keratins was examined in 100 OSCC and oral epithelial dysplasia (OED) cases. Many changes in keratin expression were observed and, significantly, consistent down-regulation of keratin 4 (K4) and K13 expression was observed. Aberrant expression of K4 and K13 was associated with morphological changes in the affected oral epithelium. Experiments with cell cultures transfected with various keratin subtypes suggested that alterations in keratin subtype expression can cause changes in cell shape and movement. CONCLUSIONS: Aberrant expression of K4 and K13, which are the dominant pair of differentiation-related keratins in oral keratinocytes, indicates dysregulation of epithelial differentiation in OSCC and OED. These keratins, especially K4, may be useful for pathological diagnosis. We propose that the aberrant expression of K4 and K13 and concomitant up-regulation of the other keratins may be one of the causative factors for morphological alterations in the affected epithelium.
AIMS: This study aimed to identify relevant keratin subtypes that may associate with the pathogenesis of oral epithelial neoplasms. METHODS AND RESULTS: Expression of all the keratin subtypes was examined by cDNA microarray analysis of 43 oral squamous cell carcinoma (OSCC) cases. Immunohistochemical expression of the major keratins was examined in 100 OSCC and oral epithelial dysplasia (OED) cases. Many changes in keratin expression were observed and, significantly, consistent down-regulation of keratin 4 (K4) and K13 expression was observed. Aberrant expression of K4 and K13 was associated with morphological changes in the affected oral epithelium. Experiments with cell cultures transfected with various keratin subtypes suggested that alterations in keratin subtype expression can cause changes in cell shape and movement. CONCLUSIONS: Aberrant expression of K4 and K13, which are the dominant pair of differentiation-related keratins in oral keratinocytes, indicates dysregulation of epithelial differentiation in OSCC and OED. These keratins, especially K4, may be useful for pathological diagnosis. We propose that the aberrant expression of K4 and K13 and concomitant up-regulation of the other keratins may be one of the causative factors for morphological alterations in the affected epithelium.
Authors: Mara Gilardi; Zhiyong Wang; Marco Proietto; Anastasia Chillà; Juan Luis Calleja-Valera; Yusuke Goto; Marco Vanoni; Matthew R Janes; Zbigniew Mikulski; Antonio Gualberto; Alfredo A Molinolo; Napoleone Ferrara; J Silvio Gutkind; Francis Burrows Journal: Mol Cancer Ther Date: 2020-07-29 Impact factor: 6.261
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Authors: Dipak Sapkota; Ove Bruland; Himalaya Parajuli; Tarig A Osman; Muy-Teck Teh; Anne C Johannessen; Daniela Elena Costea Journal: BMC Cancer Date: 2015-09-09 Impact factor: 4.430
Authors: Jayalakshmi Nair; Prachi Jain; Udita Chandola; Vinayak Palve; N R Harsha Vardhan; Ram Bhupal Reddy; Vikram D Kekatpure; Amritha Suresh; Moni Abraham Kuriakose; Binay Panda Journal: Genes Cancer Date: 2015-07