| Literature DB >> 21370022 |
Kathy Brumbaugh1, Wade Johnson, Wen-Chieh Liao, Mong-Shang Lin, J P Houchins, Jeff Cooper, Steven Stoesz, Roberto Campos-Gonzalez.
Abstract
Protein phosphorylation is a universal key posttranslational modification that affects the activity and other properties of intracellular proteins. Phosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is key for their use in proteomics and profiling of disease.Mesh：
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Year: 2011 PMID： 21370022 DOI： 10.1007/978-1-61779-024-9_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745