BACKGROUND: With the development of cell-based gene transfer techniques, genetically modified human keratinocytes (Kc) and fibroblasts (Fb) have been proven to be a better choice in wound repair. METHODS: This study was designed to construct in one step a gene-modified artificial skin by a genetically engineered Kc expressing PDGF-BB and Fb expressing VEGF(165) and bFGF. The wound healing effect in a full-thickness wound model was then observed. Unmodified artificial skin served as control. On the post-operative days 7, 14, and 21, residual wound area was calculated and skin wound tissues were subjected to biopsy for further investigation. RESULTS: Compared with unmodified artificial skin, gene-modified artificial skin resulted in a reduced wound contraction and a well-organized human epidermis and better formed dermis. CONCLUSIONS: The results suggest that our two-layer, gene-modified artificial skin improved both vascularization and epidermalization for skin regeneration. This technique could bring about a new approach in the treatment of burns and chronic wounds.
BACKGROUND: With the development of cell-based gene transfer techniques, genetically modified human keratinocytes (Kc) and fibroblasts (Fb) have been proven to be a better choice in wound repair. METHODS: This study was designed to construct in one step a gene-modified artificial skin by a genetically engineered Kc expressing PDGF-BB and Fb expressing VEGF(165) and bFGF. The wound healing effect in a full-thickness wound model was then observed. Unmodified artificial skin served as control. On the post-operative days 7, 14, and 21, residual wound area was calculated and skin wound tissues were subjected to biopsy for further investigation. RESULTS: Compared with unmodified artificial skin, gene-modified artificial skin resulted in a reduced wound contraction and a well-organized human epidermis and better formed dermis. CONCLUSIONS: The results suggest that our two-layer, gene-modified artificial skin improved both vascularization and epidermalization for skin regeneration. This technique could bring about a new approach in the treatment of burns and chronic wounds.
Authors: Ruben Y Kannan; Henryk J Salacinski; Kevin Sales; Peter Butler; Alexander M Seifalian Journal: Biomaterials Date: 2005-05 Impact factor: 12.479
Authors: Anna T Grazul-Bilska; Mary Lynn Johnson; Jerzy J Bilski; Dale A Redmer; Lawrence P Reynolds; Ahmed Abdullah; Kay M Abdullah Journal: Drugs Today (Barc) Date: 2003-10 Impact factor: 2.245
Authors: Jan Jeroen Vranckx; Daniela Hoeller; Patrik E M Velander; Christoph F P Theopold; Nicola Petrie; Akira Takedo; Elof Eriksson; Feng Yao Journal: Wound Repair Regen Date: 2007 Sep-Oct Impact factor: 3.617
Authors: Ziyang Zhang; Alex Slobodianski; Astrid Arnold; Jessica Nehlsen; Ursula Hopfner; Arndt F Schilling; Tatjana Perisic; Hans-Günther Machens Journal: Int J Med Sci Date: 2017-07-19 Impact factor: 3.738