PURPOSE: The aim of this study was to evaluate the complication rates and inflammatory response in TachoSil™-sealed small-diameter anastomoses with conventional and reduced suture number as a model for neonatal bowel surgery. METHODS: Ileo-ileal anastomoses were performed in 73 rats. In the control group, the anastomosis was accomplished with the conventional technique, using nine interrupted sutures. In the other groups with nine, six, and three interrupted sutures, the anastomotic line was additionally sealed with a fibrin-coated collagen patch (TachoSil™). The rats were sacrificed on days 0, 2, and 10. Clinical and functional parameters included the rates of ileus, insufficiency and death, operating time, adhesions, bursting pressure, and preanastomotic dilatation. The histological examination of the anastomoses concentrated on assessing the inflammatory cell infiltration of the TachoSil™ patch and the intestinal wall. RESULTS: Severe preanastomotic dilatation was observed in additionally sealed ileo-ileal anastomoses with conventional suture number and high complication rates (ileus, perforation, death) occurred in additionally sealed anastomoses with reduced suture number. We found a massive microabscess-forming inflammation in additionally sealed anastomoses. Inflammatory cell infiltration was highest in the collagen layer of the sealing patch (p < 0.05 vs. fibrin layer of the sealing patch and vs. intestinal wall). CONCLUSIONS: As a result of our findings, additional sealing of small-diameter intestinal anastomoses with TachoSil™ cannot be recommended.
PURPOSE: The aim of this study was to evaluate the complication rates and inflammatory response in TachoSil™-sealed small-diameter anastomoses with conventional and reduced suture number as a model for neonatal bowel surgery. METHODS: Ileo-ileal anastomoses were performed in 73 rats. In the control group, the anastomosis was accomplished with the conventional technique, using nine interrupted sutures. In the other groups with nine, six, and three interrupted sutures, the anastomotic line was additionally sealed with a fibrin-coated collagen patch (TachoSil™). The rats were sacrificed on days 0, 2, and 10. Clinical and functional parameters included the rates of ileus, insufficiency and death, operating time, adhesions, bursting pressure, and preanastomotic dilatation. The histological examination of the anastomoses concentrated on assessing the inflammatory cell infiltration of the TachoSil™ patch and the intestinal wall. RESULTS: Severe preanastomotic dilatation was observed in additionally sealed ileo-ileal anastomoses with conventional suture number and high complication rates (ileus, perforation, death) occurred in additionally sealed anastomoses with reduced suture number. We found a massive microabscess-forming inflammation in additionally sealed anastomoses. Inflammatory cell infiltration was highest in the collagen layer of the sealing patch (p < 0.05 vs. fibrin layer of the sealing patch and vs. intestinal wall). CONCLUSIONS: As a result of our findings, additional sealing of small-diameter intestinal anastomoses with TachoSil™ cannot be recommended.
Authors: Kamacay Cira; Felix Stocker; Stefan Reischl; Andreas Obermeier; Helmut Friess; Rainer Burgkart; Philipp-Alexander Neumann Journal: Front Surg Date: 2022-04-22
Authors: Rozemarijn J van der Vijver; Cees J H M van Laarhoven; Ben M de Man; Roger M L M Lomme; Thijs Hendriks Journal: Int J Colorectal Dis Date: 2012-03-08 Impact factor: 2.571