BACKGROUND: Recent evidence suggests that the duodenum can regulate iron absorption independently of hepcidin via the transcription factor Hif-2α acting directly on the transcription of the proteins involved in the iron transport. The current study investigates the temporal relationship between Dcytb and Hif-2α during early hypoxic stimulus in the enterocyte in vivo. METHODS: Duodenal Dcytb and Hif-2α protein expression was analysed by Western blot technique while gene regulation was determined by quantitative PCR. RESULTS: Both Dcytb and Hif-2α protein expression were increased during the first hours of hypoxic duration. A change in hepcidin expression however, was significant only at 72 h hypoxia. Increased iron absorption reported in early hypoxia could be accounted for in part by the enhancement of Dcytb expression by Hif-2α in the duodenum. CONCLUSION: Modulation of Hif-2α predominates over hepcidin in the regulation of intestinal iron absorption during short hypoxic duration. The intestine exerts regulatory mechanisms in the dietary absorption of iron into systemic circulation.
BACKGROUND: Recent evidence suggests that the duodenum can regulate iron absorption independently of hepcidin via the transcription factor Hif-2α acting directly on the transcription of the proteins involved in the iron transport. The current study investigates the temporal relationship between Dcytb and Hif-2α during early hypoxic stimulus in the enterocyte in vivo. METHODS: Duodenal Dcytb and Hif-2α protein expression was analysed by Western blot technique while gene regulation was determined by quantitative PCR. RESULTS: Both Dcytb and Hif-2α protein expression were increased during the first hours of hypoxic duration. A change in hepcidin expression however, was significant only at 72 h hypoxia. Increased iron absorption reported in early hypoxia could be accounted for in part by the enhancement of Dcytb expression by Hif-2α in the duodenum. CONCLUSION: Modulation of Hif-2α predominates over hepcidin in the regulation of intestinal iron absorption during short hypoxic duration. The intestine exerts regulatory mechanisms in the dietary absorption of iron into systemic circulation.
Authors: Ken Takeuchi; Ingvar Bjarnason; Abas H Laftah; Gladys O Latunde-Dada; Robert J Simpson; Andrew T McKie Journal: Scand J Gastroenterol Date: 2005-02 Impact factor: 2.423
Authors: David M Frazer; Sarah J Wilkins; Erika M Becker; Christopher D Vulpe; Andrew T McKie; Deborah Trinder; Gregory J Anderson Journal: Gastroenterology Date: 2002-09 Impact factor: 22.682
Authors: Pavle Matak; Sara Zumerle; Maria Mastrogiannaki; Souleiman El Balkhi; Stephanie Delga; Jacques R R Mathieu; François Canonne-Hergaux; Joel Poupon; Paul A Sharp; Sophie Vaulont; Carole Peyssonnaux Journal: PLoS One Date: 2013-03-28 Impact factor: 3.240