PURPOSE: The bone marrow microenvironment is considered a critical component in the dissemination and fate of cancer cells in the metastatic process. We explored the possible correlation between bone marrow mesenchymal stem cells (BM-MSC) and disseminated breast cancer-initiating cells (BCIC) in primary breast cancer patients. EXPERIMENTAL DESIGN: Bone marrow mononuclear cells (BM-MNC) were collected at the time of primary surgery in 12 breast cancer patients. BM-MNC was immunophenotyped and BCIC was defined as epithelial cells (CD326+CD45-) with a "stem-like" phenotype (CD44+CD24low/-, ALDH activity). BM-MSC was defined as CD34-CD45-cells that co-expressed GD2, CD271, and/or CD200 within CD326-depleted BM-MNC. RESULTS: The percentages of BCIC (Aldefluor+CD326+CD44+CD24-) correlated with the percentages of BM-MSC, either CD45-GD2+CD200+CD271+ (Kedall's tau = 0.684, p = 0.004) or CD45-GD2+CD271+ in the bone marrow (Kedall's tau = 0.464, p = 0.042). CONCLUSIONS: There was a positive correlation between mesenchymal stem cells expressing GD2 and CD271 and breast cancer-initiating cells in BM of patients with primary breast cancer.
PURPOSE: The bone marrow microenvironment is considered a critical component in the dissemination and fate of cancer cells in the metastatic process. We explored the possible correlation between bone marrow mesenchymal stem cells (BM-MSC) and disseminated breast cancer-initiating cells (BCIC) in primary breast cancerpatients. EXPERIMENTAL DESIGN: Bone marrow mononuclear cells (BM-MNC) were collected at the time of primary surgery in 12 breast cancerpatients. BM-MNC was immunophenotyped and BCIC was defined as epithelial cells (CD326+CD45-) with a "stem-like" phenotype (CD44+CD24low/-, ALDH activity). BM-MSC was defined as CD34-CD45-cells that co-expressed GD2, CD271, and/or CD200 within CD326-depleted BM-MNC. RESULTS: The percentages of BCIC (Aldefluor+CD326+CD44+CD24-) correlated with the percentages of BM-MSC, either CD45-GD2+CD200+CD271+ (Kedall's tau = 0.684, p = 0.004) or CD45-GD2+CD271+ in the bone marrow (Kedall's tau = 0.464, p = 0.042). CONCLUSIONS: There was a positive correlation between mesenchymal stem cells expressing GD2 and CD271 and breast cancer-initiating cells in BM of patients with primary breast cancer.
Authors: Bruno Delorme; Jochen Ringe; Nathalie Gallay; Yves Le Vern; Dominique Kerboeuf; Christian Jorgensen; Philippe Rosset; Luc Sensebé; Pierre Layrolle; Thomas Häupl; Pierre Charbord Journal: Blood Date: 2007-12-17 Impact factor: 22.113
Authors: Kelly E Corcoran; Katarzyna A Trzaska; Helen Fernandes; Margarette Bryan; Marcelo Taborga; Venkatesh Srinivas; Kathryn Packman; Prem S Patel; Pranela Rameshwar Journal: PLoS One Date: 2008-06-25 Impact factor: 3.240
Authors: Hong-Wei Wu; Michael A Sheard; Jemily Malvar; G Esteban Fernandez; Yves A DeClerck; Laurence Blavier; Hiroyuki Shimada; Charles P Theuer; Richard Sposto; Robert C Seeger Journal: Clin Cancer Res Date: 2019-05-08 Impact factor: 12.531
Authors: Erica L Carpenter; JulieAnn Rader; Jacob Ruden; Eric F Rappaport; Kristen N Hunter; Paul L Hallberg; Kate Krytska; Peter J O'Dwyer; Yael P Mosse Journal: Front Oncol Date: 2014-07-31 Impact factor: 6.244
Authors: Laura F Hutchins; Issam Makhoul; Peter D Emanuel; Angela Pennisi; Eric R Siegel; Fariba Jousheghany; Xueyan Guo; Anastas D Pashov; Behjatolah Monzavi-Karbassi; Thomas Kieber-Emmons Journal: Oncotarget Date: 2017-10-23
Authors: Henrietta O Fasanya; Pablo J Dopico; Zachary Yeager; Z Hugh Fan; Dietmar W Siemann Journal: J Bone Oncol Date: 2021-03-24 Impact factor: 4.491