Literature DB >> 21357166

Drosophila embryo dechorionation.

Wendy F Rothwell, William Sullivan.   

Abstract

INTRODUCTIONEarly Drosophila embryos are particularly amenable to cellular analysis. However, they are protected by an outer chorion layer, as well as an impermeable and opaque vitelline membrane. Consequently, preparation of Drosophila embryos for fixed-sample analysis must include procedures for chorion removal, vitelline membrane permeabilization, fixation, and finally, vitelline membrane removal. This protocol describes a method for removing the chorion layer.

Entities:  

Year:  2007        PMID： 21357166     DOI： 10.1101/pdb.prot4826

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  6 in total

1.  Whole mount RNA fluorescent in situ hybridization of Drosophila embryos.

Authors:  Félix Legendre; Neal Cody; Carole Iampietro; Julie Bergalet; Fabio Alexis Lefebvre; Gaël Moquin-Beaudry; Olivia Zhang; Xiaofeng Wang; Eric Lécuyer
Journal:  J Vis Exp       Date:  2013-01-30       Impact factor: 1.355

2.  Whole-Mount RNA FISH Combined with Immunofluorescence for the Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline.

Authors:  Valeriya Morgunova; Maria M Sukhova; Alla Kalmykova
Journal:  Methods Mol Biol       Date:  2022

3.  Fluorescent lectin staining of Drosophila embryos and tissues to detect the spatial distribution of glycans during development.

Authors:  E Tian; Liping Zhang; Kelly G Ten Hagen
Journal:  Methods Mol Biol       Date:  2013

4.  Circumventing heterozygosity: sequencing the amplified genome of a single haploid Drosophila melanogaster embryo.

Authors:  Charles H Langley; Marc Crepeau; Charis Cardeno; Russell Corbett-Detig; Kristian Stevens
Journal:  Genetics       Date:  2011-03-24       Impact factor: 4.562

5.  Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos.

Authors:  Eliana F Torres-Zelada; Smitha George; Hannah R Blum; Vikki M Weake
Journal:  J Cell Sci       Date:  2022-01-24       Impact factor: 5.285

6.  Intergenerational inheritance of high fat diet-induced cardiac lipotoxicity in Drosophila.

Authors:  Maria Clara Guida; Ryan Tyge Birse; Alessandra Dall'Agnese; Paula Coutinho Toto; Soda Balla Diop; Antonello Mai; Peter D Adams; Pier Lorenzo Puri; Rolf Bodmer
Journal:  Nat Commun       Date:  2019-01-14       Impact factor: 14.919
  6 in total

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