Literature DB >> 21357073

Cell Preparation and Multicolor FISH in 3D Preserved Cultured Mammalian Cells.

Marion Cremer1, Stefan Müller, Daniela Köhler, Alessandro Brero, Irina Solovei.   

Abstract

INTRODUCTIONFluorescence in situ hybridization (FISH) on three-dimensional preserved nuclei (3D-FISH) in combination with three-dimensional-microscopy and image reconstruction is an efficient tool to analyze the arrangement of distinct nuclear targets such as entire chromosome territories, chromosomal subregions, or single gene loci on a single-cell level. This protocol focuses on fixation, pretreatments, and 3D-FISH on cultured mammalian cells. It can be applied to a variety of cell types growing adherently or in suspension.

Entities:  

Year:  2007        PMID： 21357073     DOI： 10.1101/pdb.prot4723

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  13 in total

1.  Histone acetylation controls the inactive X chromosome replication dynamics.

Authors:  Corella S Casas-Delucchi; Alessandro Brero; Hans-Peter Rahn; Irina Solovei; Anton Wutz; Thomas Cremer; Heinrich Leonhardt; M Cristina Cardoso
Journal:  Nat Commun       Date:  2011       Impact factor: 14.919

2.  Lineage-specific rearrangement of chromatin loops and epigenomic features during adipocytes and osteoblasts commitment.

Authors:  Ruo-Han Hao; Yan Guo; Chen Wang; Fei Chen; Chen-Xi Di; Shan-Shan Dong; Qi-Long Cao; Jing Guo; Yu Rong; Shi Yao; Dong-Li Zhu; Yi-Xiao Chen; Hao Chen; Tie-Lin Yang
Journal:  Cell Death Differ       Date:  2022-07-29       Impact factor: 12.067

3.  Spatial organization of chromatin domains and compartments in single chromosomes.

Authors:  Siyuan Wang; Jun-Han Su; Brian J Beliveau; Bogdan Bintu; Jeffrey R Moffitt; Chao-ting Wu; Xiaowei Zhuang
Journal:  Science       Date:  2016-07-21       Impact factor: 47.728

4.  Toxoplasma gondii chromodomain protein 1 binds to heterochromatin and colocalises with centromeres and telomeres at the nuclear periphery.

Authors:  Mathieu Gissot; Robert Walker; Stephane Delhaye; Ludovic Huot; David Hot; Stanislas Tomavo
Journal:  PLoS One       Date:  2012-03-09       Impact factor: 3.240

5.  Comprehensive FISH probe design tool applied to imaging human immunoglobulin class switch recombination.

Authors:  Jakub Nedbal; Philip S Hobson; David J Fear; Rainer Heintzmann; Hannah J Gould
Journal:  PLoS One       Date:  2012-12-14       Impact factor: 3.240

6.  Reorganization of chromosome architecture in replicative cellular senescence.

Authors:  Steven W Criscione; Marco De Cecco; Benjamin Siranosian; Yue Zhang; Jill A Kreiling; John M Sedivy; Nicola Neretti
Journal:  Sci Adv       Date:  2016-02-05       Impact factor: 14.136

7.  Small chromosomal regions position themselves autonomously according to their chromatin class.

Authors:  Harmen J G van de Werken; Josien C Haan; Yana Feodorova; Dominika Bijos; An Weuts; Koen Theunis; Sjoerd J B Holwerda; Wouter Meuleman; Ludo Pagie; Katharina Thanisch; Parveen Kumar; Heinrich Leonhardt; Peter Marynen; Bas van Steensel; Thierry Voet; Wouter de Laat; Irina Solovei; Boris Joffe
Journal:  Genome Res       Date:  2017-03-24       Impact factor: 9.043

8.  PML nuclear bodies contribute to the basal expression of the mTOR inhibitor DDIT4.

Authors:  Jayme Salsman; Alex Stathakis; Ellen Parker; Dudley Chung; Livia E Anthes; Kara L Koskowich; Sara Lahsaee; Daniel Gaston; Kimberly R Kukurba; Kevin S Smith; Ian C Chute; Daniel Léger; Laura D Frost; Stephen B Montgomery; Stephen M Lewis; Christopher Eskiw; Graham Dellaire
Journal:  Sci Rep       Date:  2017-03-23       Impact factor: 4.379

Review 9.  Closing the loop: 3C versus DNA FISH.

Authors:  Luca Giorgetti; Edith Heard
Journal:  Genome Biol       Date:  2016-10-19       Impact factor: 13.583

10.  Persistent infection by HSV-1 is associated with changes in functional architecture of iPSC-derived neurons and brain activation patterns underlying working memory performance.

Authors:  Leonardo D'Aiuto; Konasale M Prasad; Catherine H Upton; Luigi Viggiano; Jadranka Milosevic; Giorgio Raimondi; Lora McClain; Kodavali Chowdari; Jay Tischfield; Michael Sheldon; Jennifer C Moore; Robert H Yolken; Paul R Kinchington; Vishwajit L Nimgaonkar
Journal:  Schizophr Bull       Date:  2014-03-12       Impact factor: 9.306
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