Literature DB >> 21357029

Culture of Drosophila: the laboratory setup.

Michael Ashburner, John Roote.   

Abstract

INTRODUCTIONSince its introduction to experimental biology more than 90 years ago, Drosophila melanogaster has proved to be an easily cultured and robust laboratory animal. Although culture techniques and the ways in which flies are handled have changed over the years, if he were to enter a fly room today, T.H. Morgan would clearly recognize what is being done, and why. This article provides the basic methods for the laboratory culture of D. melanogaster. The intelligent culture of Drosophila requires a basic understanding of the life cycle of this fly. For this reason, we begin by describing the life cycle of Drosophila and then present information for setting up a fly laboratory.

Entities:  

Year:  2007        PMID: 21357029     DOI: 10.1101/pdb.ip34

Source DB:  PubMed          Journal:  CSH Protoc        ISSN: 1559-6095


  3 in total

1.  Live cell cycle analysis of Drosophila tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle violet DNA stain.

Authors:  Kerry Flegel; Dan Sun; Olga Grushko; Yiqin Ma; Laura Buttitta
Journal:  J Vis Exp       Date:  2013-05-19       Impact factor: 1.355

2.  Mutant human torsinA, responsible for early-onset dystonia, dominantly suppresses GTPCH expression, dopamine levels and locomotion in Drosophila melanogaster.

Authors:  Noriko Wakabayashi-Ito; Rami R Ajjuri; Benjamin W Henderson; Olugbenga M Doherty; Xandra O Breakefield; Janis M O'Donnell; Naoto Ito
Journal:  Biol Open       Date:  2015-04-17       Impact factor: 2.422

3.  Maintenance of a Drosophila melanogaster Population Cage.

Authors:  Juan Manuel Caravaca; Elissa P Lei
Journal:  J Vis Exp       Date:  2016-03-15       Impact factor: 1.355

  3 in total

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