SDS-PAGE Peptide-Mapping Procedure (Cleveland Method).

INTRODUCTIONThe Cleveland method for peptide mapping of proteins relies on partial hydrolysis of the protein yielding a considerable number of large peptides that can be readily identified by SDS-PAGE or tricine-SDS-PAGE. Small peptides obtained with a total digest are often poorly fixed within the gel and usually contribute nothing to the peptide map. In the method described here, the protein is partially digested in solution, and the resulting peptide fragments are separated by SDS-PAGE.