Literature DB >> 21356980

Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis.

Amber L Wells1, John S Condeelis, Robert H Singer, Daniel Zenklusen.   

Abstract

INTRODUCTIONThis protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced.

Year:  2007        PMID： 21356980      PMCID： PMC4963622          DOI： 10.1101/pdb.prot4872

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  3 in total

1.  Precise nanometer localization analysis for individual fluorescent probes.

Authors:  Russell E Thompson; Daniel R Larson; Watt W Webb
Journal:  Biophys J       Date:  2002-05       Impact factor: 4.033

2.  Single mRNA molecules demonstrate probabilistic movement in living mammalian cells.

Authors:  Dahlene Fusco; Nathalie Accornero; Brigitte Lavoie; Shailesh M Shenoy; Jean-Marie Blanchard; Robert H Singer; Edouard Bertrand
Journal:  Curr Biol       Date:  2003-01-21       Impact factor: 10.834

3.  Sizing-up finite fluorescent particles with nanometer-scale precision by convolution and correlation image analysis.

Authors:  Arne Gennerich; Detlev Schild
Journal:  Eur Biophys J       Date:  2004-12-18       Impact factor: 1.733
  3 in total

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