Literature DB >> 21356978

Imaging real-time gene expression in living yeast.

Daniel Zenklusen1, Amber L Wells, John S Condeelis, Robert H Singer.   

Abstract

INTRODUCTIONThis protocol describes the application of the MS2 system to the yeast Saccharomyces cerevisiae. ASH1 mRNA tagged with six MS2 repeats (6MBSs) is used to follow the localization of the ASH1 mRNA particles to the bud tip of a haploid yeast cell. W303 yeast cells transformed with pG14-MS2-GFP and pGAL-lacZ-MS2-ASH1 are grown on select medium lacking tryptophan and leucine. RNA expression is induced by the addition of galactose, and a time-lapse movie is then acquired.

Entities:  

Year:  2007        PMID： 21356978      PMCID： PMC4956917          DOI： 10.1101/pdb.prot4870

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  2 in total

1.  Localization and anchoring of mRNA in budding yeast.

Authors:  D L Beach; E D Salmon; K Bloom
Journal:  Curr Biol       Date:  1999-06-03       Impact factor: 10.834

2.  Localization of ASH1 mRNA particles in living yeast.

Authors:  E Bertrand; P Chartrand; M Schaefer; S M Shenoy; R H Singer; R M Long
Journal:  Mol Cell       Date:  1998-10       Impact factor: 17.970
  2 in total
  5 in total

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2.  Context-dependent deposition and regulation of mRNAs in P-bodies.

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Journal:  Elife       Date:  2018-01-03       Impact factor: 8.140

3.  Simple rules for passive diffusion through the nuclear pore complex.

Authors:  Benjamin L Timney; Barak Raveh; Roxana Mironska; Jill M Trivedi; Seung Joong Kim; Daniel Russel; Susan R Wente; Andrej Sali; Michael P Rout
Journal:  J Cell Biol       Date:  2016-10-03       Impact factor: 10.539

4.  Dynamic imaging demonstrates that pulsed electromagnetic fields (PEMF) suppress IL-6 transcription in bovine nucleus pulposus cells.

Authors:  Xinyan Tang; Tamara Alliston; Dezba Coughlin; Stephanie Miller; Nianli Zhang; Erik I Waldorff; James T Ryaby; Jeffrey C Lotz
Journal:  J Orthop Res       Date:  2017-10-17       Impact factor: 3.494

5.  The Fragile X mental retardation protein regulates matrix metalloproteinase 9 mRNA at synapses.

Authors:  Aleksandra Janusz; Jacek Milek; Malgorzata Perycz; Laura Pacini; Claudia Bagni; Leszek Kaczmarek; Magdalena Dziembowska
Journal:  J Neurosci       Date:  2013-11-13       Impact factor: 6.167
  5 in total

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