Literature DB >> 21356976

In situ hybridization of whole-mount mouse embryos with RNA probes: hybridization, washes, and histochemistry.

Thomas Lufkin.   

Abstract

INTRODUCTIONThis is the second of two protocols describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate.

Entities:  

Year:  2007        PMID： 21356976     DOI： 10.1101/pdb.prot4823

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  5 in total

1.  Midline craniofacial malformations with a lipomatous cephalocele are associated with insufficient closure of the neural tube in the tuft mouse.

Authors:  Keith S K Fong; Dana A T Adachi; Shaun B Chang; Scott Lozanoff
Journal:  Birth Defects Res A Clin Mol Teratol       Date:  2014-06-13

2.  The bowfin genome illuminates the developmental evolution of ray-finned fishes.

Authors:  Andrew W Thompson; M Brent Hawkins; Elise Parey; Dustin J Wcisel; Tatsuya Ota; Kazuhiko Kawasaki; Emily Funk; Mauricio Losilla; Olivia E Fitch; Qiaowei Pan; Romain Feron; Alexandra Louis; Jérôme Montfort; Marine Milhes; Brett L Racicot; Kevin L Childs; Quenton Fontenot; Allyse Ferrara; Solomon R David; Amy R McCune; Alex Dornburg; Jeffrey A Yoder; Yann Guiguen; Hugues Roest Crollius; Camille Berthelot; Matthew P Harris; Ingo Braasch
Journal:  Nat Genet       Date:  2021-08-30       Impact factor: 38.330

3.  The Ribosomal Protein L5 Functions During Xenopus Anterior Development Through Apoptotic Pathways.

Authors:  Corinna Schreiner; Bianka Kernl; Petra Dietmann; Ricarda J Riegger; Michael Kühl; Susanne J Kühl
Journal:  Front Cell Dev Biol       Date:  2022-02-22

4.  Functions of block of proliferation 1 during anterior development in Xenopus laevis.

Authors:  Corinna Gärtner; Annika Meßmer; Petra Dietmann; Michael Kühl; Susanne J Kühl
Journal:  PLoS One       Date:  2022-08-25       Impact factor: 3.752

5.  A p53-dependent translational program directs tissue-selective phenotypes in a model of ribosomopathies.

Authors:  Gerald C Tiu; Craig H Kerr; Craig M Forester; Pallavi S Krishnarao; Hannah D Rosenblatt; Nitin Raj; Travis C Lantz; Olena Zhulyn; Margot E Bowen; Leila Shokat; Laura D Attardi; Davide Ruggero; Maria Barna
Journal:  Dev Cell       Date:  2021-07-08       Impact factor: 13.417
  5 in total

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