Literature DB >> 21356954

Transposon-mediated transgenesis in rats.

Chuan-Wei Jang1, Richard R Behringer.   

Abstract

INTRODUCTIONThis protocol describes a method for the production of transgenic rats by coinjecting circular PiggyBac transposon-containing plasmid DNA with transposase-encoding mRNA. After fertilized eggs are collected from females, pronuclei are microinjected with the DNA and mRNA and then transferred immediately into the oviducts of pseudopregnant foster females. Using this procedure, we have obtained transformation frequencies of 33%-100% (average of ~80%). Traditional methods for generating transgenic rats (i.e., microinjection of linear DNA) yield 3%-41% transgenic founders. Thus, transposon-mediated transformation of fertilized rat eggs is an efficient alternative method to generate transgenic rats.

Entities:  

Year:  2007        PMID： 21356954     DOI： 10.1101/pdb.prot4866

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  9 in total

Review 1.  Modeling human neurodegenerative diseases in transgenic systems.

Authors:  Miguel A Gama Sosa; Rita De Gasperi; Gregory A Elder
Journal:  Hum Genet       Date:  2011-12-14       Impact factor: 4.132

Review 2.  Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.

Authors:  Pablo Bosch; Diego O Forcato; Fabrisio E Alustiza; Ana P Alessio; Alejandro E Fili; María F Olmos Nicotra; Ana C Liaudat; Nancy Rodríguez; Thirumala R Talluri; Wilfried A Kues
Journal:  Cell Mol Life Sci       Date:  2015-02-01       Impact factor: 9.261

3.  Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat.

Authors:  Kenryo Furushima; Chuan-Wei Jang; Diane W Chen; Ningna Xiao; Paul A Overbeek; Richard R Behringer
Journal:  Genetics       Date:  2012-09-28       Impact factor: 4.562

4.  DNA-PK facilitates piggyBac transposition by promoting paired-end complex formation.

Authors:  Yan Jin; Yaohui Chen; Shimin Zhao; Kun-Liang Guan; Yuan Zhuang; Wenhao Zhou; Xiaohui Wu; Tian Xu
Journal:  Proc Natl Acad Sci U S A       Date:  2017-06-23       Impact factor: 11.205

Review 5.  piggyBac-ing models and new therapeutic strategies.

Authors:  Lauren E Woodard; Matthew H Wilson
Journal:  Trends Biotechnol       Date:  2015-07-23       Impact factor: 19.536

6.  Chimeric piggyBac transposases for genomic targeting in human cells.

Authors:  Jesse B Owens; Johann Urschitz; Ilko Stoytchev; Nong C Dang; Zoia Stoytcheva; Mahdi Belcaid; Kommineni J Maragathavally; Craig J Coates; David J Segal; Stefan Moisyadi
Journal:  Nucleic Acids Res       Date:  2012-04-09       Impact factor: 16.971

7.  Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.

Authors:  Nils-Holger Zschemisch; Silke Glage; Dirk Wedekind; Edward J Weinstein; Xiaoxia Cui; Martina Dorsch; Hans-Jürgen Hedrich
Journal:  BMC Immunol       Date:  2012-11-08       Impact factor: 3.615

8.  Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon.

Authors:  Tianda Li; Ling Shuai; Junjie Mao; Xuepeng Wang; Mei Wang; Xinxin Zhang; Leyun Wang; Yanni Li; Wei Li; Qi Zhou
Journal:  Sci Rep       Date:  2016-09-14       Impact factor: 4.379

9.  A novel piggyBac transposon inducible expression system identifies a role for AKT signalling in primordial germ cell migration.

Authors:  James D Glover; Lorna Taylor; Adrian Sherman; Caroline Zeiger-Poli; Helen M Sang; Michael J McGrew
Journal:  PLoS One       Date:  2013-11-04       Impact factor: 3.240
  9 in total

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